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Ictotest® Reagent Tablets are used to test for the presence of bilirubin in urine. The presence of bilirubin is an important finding in the evaluation of liver function and a positive detection could be an indication of liver disease such as jaundice, cirrhosis or hepatitis. Hospital and laboratories rely on the quality of Siemens’ Ictotest reagent tablets to confirm bilirubin results prior to providing the results to their customers.
- Ictotest Reagent Tablets were first made available in 1953
- Use of Ictotest requires a dropper and distilled water, in addition to the absorbent mats and reagent tablets provided.
- The test is easy to perform and gives timely and accurate confirmatory urine bilirubin results in one minute.
- Level of detection ≥ 0.05 to 0.1 mg bilirubin/dL in urine (0.9–1.7 µmol/L).
Features & Benefits
Why is a confirmatory test necessary for urinary bilirubin? To date, there is no quantitative urine bilirubin test method on the market. The only way to rule out urine-strip false-positive bilirubin results is with a confirmatory assay such as Ictotest tablets. The alternative is for the healthcare professional to order a blood test for bilirubin, which adds cost and time to the confirmation process.
Use of Ictotest tablets require a pipette and distilled water, in addition to the absorbent mats and reagent tablets provided with the Ictotest kit. The procedure is easy to perform and gives reliable answers when a fresh urine specimen is used and the tablets are properly stored.
The Ictotest tablets, based on the diazotization reaction, are utilized as a confirmatory assay for the presence of urinary bilirubin when a frontline test (i.e. urine strip or another semi-quantitative method) has provided a positive bilirubin result. The Ictotest reagent tablets can be used to rule-out the presence of interfering substances that may cause a false positive result.
- Place a square of the absorbent test mat supplied onto a paper towel. Using either side of the test mat will produce expected results. Please 10 drops of urine onto the center of the test mat.
- Shake one Ictotest Reagent Tablet into the the bottle cap and transfer the tablet to the center of the moistened mat. Do not handle tablet with your fingers. Recap the bottle promptly.
- Place one drop of distilled water onto the tablet. Wait 5 seconds, then place a second drop of distilled water onto the tablet so that the distilled water runs off the tablet onto the mat.
- Observe the color of the mat around and under the tablet at 60 seconds.
RESULTS: Results with Ictotest Reagent Tablets are negative if no blue or purple color develops on the mat within 60 seconds. If a blue or purple color develops on the mat or under the tablet within 60 seconds, the result is positive. Pink or red color should be ignored.
POSITIVE RESULT: The presence of a blue or purple color on the mat indicates that bilirubin is present. The illustration at left shows typical positive results. The color gradient shows a sample range of color for a positive result.
NEGATIVE RESULTS: The absence of blue or purple color on the mat indicates that no bilirubin is present. A slight pink or red color should be ignored. The illustration at left shows typical negative results. The color gradient shows a sample range of color for a negative result.