Viscosizer TD Automating Low Volume Viscosity Analysis

Malvern Panalytical Viscosizer TD is an automated biophysical characterization tool utilizing Taylor Dispersion Analysis and providing ultra-low volume, solution-based molecular size and stability measurement capabilities, combined with Poiseuille flow for relative viscosity assessment.

Viscosizer TD offers biopharmaceutical researchers an orthogonal technique for conformational stability analysis, which is unrivaled in extending into label-free measurements in highly complex solutions, and encompasses a critical size range from small molecules through to protein-based systems.

Viscosizer TD is unique in using Taylor Dispersion Analysis with UV area imaging in microcapillary flow to enable ultra-low volume biophysical characterization of target biomolecules in solution.

Taylor Dispersion Analysis is an inherently simple experimental method, whereby a nanoliter volume sample pulse is injected into a laminar flow of matched buffer. Analysis of the time-evolved concentration profile (or Taylorgram) from UV absorption allows the molecular diffusion coefficient and hence hydrodynamic radius of solute molecules to be determined.

Viscosizer TD uses fully automated methodology to analyze stability of small molecules, peptides and proteins, and samples with mixtures of these species, even in the presence of excipients and surfactants that can be the source of measurement interference in other techniques.

Microcapillary flow methodology also allows complementary relative viscosity measurement on the same sample using Poiseuille’s law.

  • Viscosizer TD is an easy to use system with sample storage and presentation in a temperature-controlled autosampler to ensure sample integrity
  • Flexible and intuitive software interface provides fully automated test schedules and data analyses for walk-away operation
  • 40 nanoliter sample volume for molecular sizing and 6 microliter sample volume for relative viscosity
  • Total sample volume of 35 microliters required for triplicate measurements for both size and relative viscosity on one sample (15 microliter excess is recoverable)
  • UV detection for molecular sizing allows measurements at low concentration down to microgram quantities
  • Relative viscosity measurements with unique dual pass timing provides high resolution measurements with high reproducibility at low concentration and low viscosity
  • Taylor Dispersion Analysis spans an unmatched molecular size range in solution, from small molecule size, peptide size and protein size, and samples with mixtures of these species
  • Orthogonal technique for protein stability assay and protein solubility studies from candidate validation into early stage formulation development
  • Novel technique for biopharmaceutical researchers to assess peptide stability and peptide solubility
  • UV wavelength can be changed to optimize measurement sensitivity and molecular selectivity
  • Using a matched sample buffer effectively renders excipients and surfactants invisible, enabling label-free characterization of target biomolecules in complex solutions
  • Measurements are not adversely affected by the presence of a small amount of aggregates, meaning samples can be run with minimal sample preparation and without dilution or filtration

Viscosizer TD is a biophysical characterization tool that provides unique measurement capabilities for researchers assessing conformational stability, self-association and solubility of target biomolecules in complex solutions. Key application areas include:

  • Screening for developability of mAbs at early stages in the development pipeline, with ultra-low sample volume and low protein concentration
  • Optimization of lead candidates in complex buffers during formulation development
  • Assessment of Critical Quality Attributes (CQA) for biotherapeutics
  • Conducting biophysical studies of peptide-based therapeutics at low concentration
  • Advancing understanding of drug behavior at near in vivo conditions

For more information, please visit Malvern Panalytical.