Vutara 352 Super-Resolution Fluorescence Microscope by Bruker Nano Surfaces

The fastest super-resolution microscope on the market. Image deep 3D sections on live cells quickly and easily.

The Vutara 352 is the first and only system of its kind:

  • Video-rate, single-molecule localization microscopy
  • Live-cell, super-resolution protocols and workflows
  • Simultaneous four-color super-resolution imaging (including a 750 nm excitation option)
  • Turn voxels into information with quantitative analysis modules
  • Optional high-speed confocal scanner for correlative imaging
  • All raw data is stored and available in a non-proprietary format for inspection and novel analysis
  • Comprehensive online protocol and support library to speed you to publication

Applications

Live-cell imaging requires high frame rates to “freeze” cellular activity at nanoscale dimensions. Due to slow speeds, most super-resolution microscopes (STED, STORM, PALM) only produce blurred live-cell results, if any.

Four-color simultaneous (in 2D) super-resolution imaging, including excitation options ranging from 488 nm to 750 nm, provide the biologist with the required versatility to use the full range of modern genetic dyes for meaningful live-cell multicolor experiments (488 nm, 561 nm, 640 nm recommended, two-color in 3D).

HeLa cell, Green: ATTO 488 tubulin, Red: Alexa 647 midbody protein, Blue: Cy3B midbody protein.
HeLa cell, Green: ATTO 488 tubulin, Red: Alexa 647 midbody protein, Blue: Cy3B midbody protein. Courtest of Bruker Nano Surfaces

The Vutara 352 is compatible with a wide range of traditional light microscopy functionality, including swept-field / spinning disk confocal, TIRF, and other optical port accessories commonly used in live-cell imaging applications.

Best-in-class 3D imaging using Bruker’s proprietary biplane™ imaging. The biplane approach is superior to astigmatism, resulting in more accurate localization precision. Unlike most super-resolution systems (STORM, PALM, GSD), the Vutara 352 enables accurate single molecule localizations without TIRF.

The Vutara 352 uniformly illuminates the region of interest for consistent and reliable super-resolution across the whole field of view.

All raw data is stored and available in a non-proprietary format for inspection and novel analysis.

Self-contained, ocular-free enclosure minimizes thermal and vibrational imaging artifacts.

Left: AF488 - Alphatubulin (competitors system, notice inconsistent photoswitching in area A versus area B), Right: BSC1 cell AF647 - Alphatubilin (notice the whole field of view has consistent photoswitching)

Left: AF488 - Alphatubulin (competitors system, notice inconsistent photoswitching in area A versus area B), Right: BSC1 cell AF647 - Alphatubilin (notice the whole field of view has consistent photoswitching)

Technical Specifications

Overview

3D video-rate, super-resolution single-molecule localization imaging

Lateral Resolution

20 nm*

Axial Resolution

40 nm to 70 nm*

Acquisition Speed

Up to 3,000 fps

Cameras (two included)

sCMOS camera (4 MP, 6.5 µm x 6.5 µm pixel size for super-resolution imaging); CCD camera (1392 x 1040 for widefield imaging)

Imaging Depth

Up to 20 µm* via high-precision piezo Z stage (100 µm option with select samples)

Stage

Fully motorized XY scanning stage

Piezo

Objective-mounted piezo for rapid Z focus (100 µm or 400 µm travel option)

Imaging Modes

Simultaneous two-color; interleaved multi-color; sequential multi-color; ultra-fast particle tracking; wide-field imaging; transillumination

Field of View

Wide-field: 200 µm x 160 µm;
Super-resolution: 20 µm x 20 µm, optional 40 µm x 40 µm;
Single-molecule particle tracking: 20 µm x 10 µm x 1 µm, optional 40 µm x 40 µm;
Confocal: 82.2 µm x 82.2 µm

Imaging

Simultaneous two- or four-channel imaging;
Standard Quadfield™ module, simultaneous orange/red or green/orange;
Custom Quadfield module, four-color simultaneous (2D)

3D Particle Tracking

<10 nm precision*;
20 µm x 10 µm x 1µm volume at up to 3,000 fps

Laser Options

100 mW: 405 nm;
1000 mW: 488 nm, 561 nm, 640 nm, 750 nm, optional 532 nm

Objectives

Water: 60x/1.2;
Silicone: 60x/1.3;
Oil: 60x/1.42, 60x/1.49, 100x/1.49, 150x/1.49

Software Features

Simple step-by-step "wizard" acquisition; multi-threaded real-time localization; full 3D rendering; multimodal drift correction including fiduciary drift correction; Vutara Viewer; non-proprietary export

Z Stacks

Acquisitions up to 15 µm of Z stacks via high-precision Z stage

Custom Stand

Drift-minimizing inverted microscope stand; integrated thermal control for sample temperature stability (200°C to 37°C); environmental control

Filter Wheels

2 filter wheels included

Modular Options

Modular options available upon request (e.g., Opterra SR confocal scanner, TIRF, industry-standard side port)