The fastest super-resolution microscope on the market. Image deep 3D sections on live cells quickly and easily.
The Vutara 352 is the first and only system of its kind:
- Video-rate, single-molecule localization microscopy
- Live-cell, super-resolution protocols and workflows
- Simultaneous four-color super-resolution imaging (including a 750 nm excitation option)
- Turn voxels into information with quantitative analysis modules
- Optional high-speed confocal scanner for correlative imaging
- All raw data is stored and available in a non-proprietary format for inspection and novel analysis
- Comprehensive online protocol and support library to speed you to publication
Applications
Live-cell imaging requires high frame rates to “freeze” cellular activity at nanoscale dimensions. Due to slow speeds, most super-resolution microscopes (STED, STORM, PALM) only produce blurred live-cell results, if any.
Four-color simultaneous (in 2D) super-resolution imaging, including excitation options ranging from 488 nm to 750 nm, provide the biologist with the required versatility to use the full range of modern genetic dyes for meaningful live-cell multicolor experiments (488 nm, 561 nm, 640 nm recommended, two-color in 3D).
HeLa cell, Green: ATTO 488 tubulin, Red: Alexa 647 midbody protein, Blue: Cy3B midbody protein. Courtest of Bruker Nano Surfaces
The Vutara 352 is compatible with a wide range of traditional light microscopy functionality, including swept-field / spinning disk confocal, TIRF, and other optical port accessories commonly used in live-cell imaging applications.
Best-in-class 3D imaging using Bruker’s proprietary biplane™ imaging. The biplane approach is superior to astigmatism, resulting in more accurate localization precision. Unlike most super-resolution systems (STORM, PALM, GSD), the Vutara 352 enables accurate single molecule localizations without TIRF.
The Vutara 352 uniformly illuminates the region of interest for consistent and reliable super-resolution across the whole field of view.
All raw data is stored and available in a non-proprietary format for inspection and novel analysis.
Self-contained, ocular-free enclosure minimizes thermal and vibrational imaging artifacts.
Left: AF488 - Alphatubulin (competitors system, notice inconsistent photoswitching in area A versus area B), Right: BSC1 cell AF647 - Alphatubilin (notice the whole field of view has consistent photoswitching)
Technical Specifications
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Overview
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3D video-rate, super-resolution single-molecule localization imaging
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Lateral Resolution
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20 nm*
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Axial Resolution
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40 nm to 70 nm*
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Acquisition Speed
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Up to 3,000 fps
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Cameras (two included)
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sCMOS camera (4 MP, 6.5 µm x 6.5 µm pixel size for super-resolution imaging); CCD camera (1392 x 1040 for widefield imaging)
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Imaging Depth
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Up to 20 µm* via high-precision piezo Z stage (100 µm option with select samples)
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Stage
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Fully motorized XY scanning stage
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Piezo
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Objective-mounted piezo for rapid Z focus (100 µm or 400 µm travel option)
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Imaging Modes
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Simultaneous two-color; interleaved multi-color; sequential multi-color; ultra-fast particle tracking; wide-field imaging; transillumination
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Field of View
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Wide-field: 200 µm x 160 µm;
Super-resolution: 20 µm x 20 µm, optional 40 µm x 40 µm;
Single-molecule particle tracking: 20 µm x 10 µm x 1 µm, optional 40 µm x 40 µm;
Confocal: 82.2 µm x 82.2 µm
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Imaging
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Simultaneous two- or four-channel imaging;
Standard Quadfield™ module, simultaneous orange/red or green/orange;
Custom Quadfield module, four-color simultaneous (2D)
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3D Particle Tracking
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<10 nm precision*;
20 µm x 10 µm x 1µm volume at up to 3,000 fps
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Laser Options
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100 mW: 405 nm;
1000 mW: 488 nm, 561 nm, 640 nm, 750 nm, optional 532 nm
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Objectives
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Water: 60x/1.2;
Silicone: 60x/1.3;
Oil: 60x/1.42, 60x/1.49, 100x/1.49, 150x/1.49
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Software Features
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Simple step-by-step "wizard" acquisition; multi-threaded real-time localization; full 3D rendering; multimodal drift correction including fiduciary drift correction; Vutara Viewer; non-proprietary export
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Z Stacks
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Acquisitions up to 15 µm of Z stacks via high-precision Z stage
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Custom Stand
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Drift-minimizing inverted microscope stand; integrated thermal control for sample temperature stability (200°C to 37°C); environmental control
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Filter Wheels
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2 filter wheels included
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Modular Options
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Modular options available upon request (e.g., Opterra SR confocal scanner, TIRF, industry-standard side port)
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