X-CLARITY™ Tissue Clearing System from Logos Biosystems

Accelerate your research with the X-CLARITY™

Sectioning is time. We cut the time.

TTime is your most valuable asset in research. Imagine the time you spend preparing, sectioning, labeling, mounting, imaging, reconstructing, and analyzing hundreds upon thousands of ultra-thin slices. Now imagine never having to section again. and disappointment.

Keep your samples whole with the X-CLARITY™ Tissue Clearing System, your all-in-one solution for electrophoretic tissue clearing. The X-CLARITY™ accelerates the removal of lipids from tissues, leaving you with structurally sound and transparent whole tissues that are ready for multiple rounds of antibody labeling and imaging.

What is clarity™?

CLARITY™ is a tissue clearing method that produces structurally sound and transparent whole tissues ready for multiple rounds of antibody labeling and imaging. The method has opened up a world of possibilities, from tracing neural circuitry to exploring the relationship between structure and function with a global perspective.

With the CLARITY method, the biomolecules in intact tissues are covalently linked to a sturdy hydrogel network, preserving molecular information and structural integrity. Lipid bilayers are broken up through electrophoresis in the presence of ionic detergents, leaving behind a stable and transparent tissue-hydrogel hybrid that is chemically accessible for molecular phenotyping.

Logos Biosystems licensed and independently developed the CLARITY technology to make significant improvements for tissue clearing efficiency and reproducibility.

How does the X-CLARITY™ work?

The X-CLARITY™ Tissue Clearing System is comprised of four components:

  • X-CLARITY™ ETC Controller
  • X-CLARITY™ ETC Chamber
  • Pump
  • Buffer reservoir

The highlights of this are:

  • Exceptional transparency
  • Fastest processing time
  • Easy installation and operation
  • Minimized artifacts
  • No leaking, no boiling
  • Pre-optimized protocol

The X-CLARITY™ has been designed to be user-friendly and efficient. Complicated wires are combined into a single cable for power supply and control. Improved electrodes generate a uniform electric field that eliminates uneven voltages and currents.

A timer allows precise control over the clearing time by automatically turning off the current at the end of the allotted time. An active cooling system minimizes the probability of artifacts arising due to poor temperature control. Buffer circulation ensures consistent buffering capacity, temperature control, and elimination of
electrophoretic byproducts.

This advanced system ensures that tissue clearing occurs efficiently and rapidly. Moreover, the X-CLARITY™ successfully produces transparent tissues that are efficiently penetrated by and labeled with macromolecules such as
antibodies or oligonucleotides. This allows for the 3D imaging of large tissues at single-cell resolution.