Automated Zebrafish screening for Myeloid Leukemia drug discovery

Thought LeadersDr Elspeth Payne & Dr  Alexandra LubinUCL Cancer Institute

In this interview, NewsMedical speaks to Elspeth Payne and Alexandra Lubin, cancer researchers at the UCL Cancer Institute in London, about how they use zebrafish modeling to study myeloid leukemias in a high-throughput, automated fashion using AI-powered analysis software, Athena. This affordable software solution is available for download in a novel pay-per-use basis. 

Could you please introduce yourself, what it is you do and briefly describe your research?

Elspeth Payne: My name is Beth Payne and I am a clinicial scientist.  My laboratory works on blood cancers, particularly myeloid malignancies, and I also look after patients in the hospital. We use a combination of zebrafish modeling and drug screens alongside primary patient material to study and better understand the development of myeloid leukemias, and different ways that we could treat them in the future.

Alexandra Lubin: My name is Alex Lubin. I am a post-doctoral research associate in Beth Payne's lab. I work on zebrafish models of myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML). Drug screening is my main project and I am looking at doing some drug screening in hematopoietic stem cells in the tails of zebrafish to look for synthetic lethality.

How did you perform analysis of zebrafish before using the Athena software?

Alexandra Lubin: When I am looking at stem cells in my drug screen, I try to measure a change in number of cells in a fish. To do this manually, all the individual fluorescent cells must be counted in the tail. That means you have to take each fish under a microscope, go through and count them, which is very time-consuming. For this reason, we started looking for an automated approach.

What are some of the challenges you face in getting the data you need?

Beth Payne: We have been developing some drug screening methodologies, and while the zebrafish is a great model for this, getting the right throughput has been challenging. Although there are many different platforms that one can use, they tend to have kind of bespoke solutions for how you analyze the data. That means that developing each new screen is both costly and it takes a long time to get something that works for your purpose.

At what point then did you move away from manual imaging and image analysis to search for automated solutions?

Beth Payne: In terms of automated screening, we have been looking for a solution for probably the last five to ten years.  We revisited this challenge every few years as a key goal, asking ourselves "how can we make this more time efficient?"  Imaging and manually counting cells is necessary for proper data quantification, but we thought that if it could be automated it would be a huge time savings.  Not only for the time needed for these steps, but also to open up a whole host of opportunities to use this sort of platform for other experiments.

What are the main challenges when sampling with a manual workflow?

Alexandra Lubin: The main challenge with the manual method is that it is time-consuming. It is not particularly difficult to do, but it involves spending hours and hours at microscopes. The other main problem with that is you cannot achieve large sample numbers. When you want to run something like a drug screen, the power comes from being able to analyze many samples.

What has this technology brought to your lab?

Beth Payne: The thing that has been helpful for this system is we use it for all indications in addition to drug screening. For example, if we have a transgenic or even an in-situ that we want to image and look at in detail in high content, we will use the Athena rather than doing it manually.

The other great thing about the system is that it is flexible and you can optimize it for a different assay relatively quickly as the system is user-friendly. The company offers a lot of support to try and help us when we run into some difficulties.

I would say that overall it allows us to power our experiments much better because instead of using just 20 embryos, we can use 200 for the same processing time. In fact, it takes considerably less time that it otherwise would have needed. Overall, it offers more flexibility into the experiments we can plan and the sort of power we can get from the data output from Athena.

AI-powered analysis of a brightfield image of a zebrafish larva to identify internal anatomy combined with detection of GFP-labelled hematopoietic stem cells.  GFP-labelled cells present in the tail, only, can be counted in a selective fashion. Image credit: IDEA Bio-Medical

Azo: When you started working on the Athena software, how long did it take you to be independent and productive with it?

Alexandra Lubin: When I first encountered the Athena software, it was very much in development. That gave room for many discussions with IDEA Bio-Medical about what we wanted the software to do, what would be useful, and highlighting areas for improvement.

Once it was up and running, the transition time from first using the software to becoming fully independent with it was relatively short.

How does that compare with other instrumentation you use, for example compared with manual microscopes?

Alexandra Lubin: This has been a unique experience in the sense that we have had more contact with IDEA Bio-Medical than we would usually have. I have never used another system like this, so I do not really have a direct comparison, but it is an excellent and extremely practical piece of software.

How long does it take you to analyze all your fish samples and how does that compare previously?

Alexandra Lubin: When conducting these experiments manually, we can only screen about a hundred fish at a time. However, that takes such a significant amount of time, but once we began using the Athena, we can increase that number.

How many fish I screen depends on how well-behaved the fish are, as anybody who works with zebrafish will know. It is possible to screen hundreds (of fish) in a short space of time as it only takes around 30 minutes between loading the plate and getting the results for about a hundred fish, whereas previously, that would have taken a whole day.

What do you do when you need some support using the software? Who supports you and how?

Alexandra Lubin: When I need some support with the software, I reach out to the team at IDEA Bio-Medical. They have been really helpful and offer a collaborative partnership. I provide them with images; they provide us with support - it works seamlessly.

What was the point when you thought the Athena software was useful to your research?

Alexandra Lubin: When I started this drug screen, we knew we needed to bring in automation as it was not going to be possible to screen thousands of compounds manually. When we started looking at different methods, it became clear how easy to use the system was and how flexible it was. Moreover, we were drawn to the fact that Athena enabled us to branch out and change it if we needed to.

Where do you see your work going next and the impact?

Beth Payne: I believe that it has certainly opened the door to performing more and more screens. Perhaps we may now be able to conduct more ambitious types of screens where we might use, for example, more than one fluorophore or more than one genotype at the same time because the time taken to screen is so much shorter and more efficient that you can introduce more variables.  

About Dr Elspeth Payne

Dr Elspeth Payne is a Senior Clinical Researcher/Clinical Consultant UCL Cancer Institute. Her laboratory at UCL’s Cancer Institute is dedicated to the study of inherited bone marrow failure disorders and leukemias, and uses zebrafish to model these diseases. Dr Payne is also a clinical hematologist at UCL Hospital, where she treats people with blood disorders including leukemia and bone marrow failure.

About Dr Alexandra Lubin

Dr. Lubin is a post-doctoral at the UCL Cancer Institute where she uses zebrafish to study myelodysplastic syndrome (MDS) and acute myeloid leukaemia (AML), aiming to develop novel therapeutic treatments. Previously, she obtained her PhD in Chemical Biology from Imperial College London after studying Chemistry at the University of Cambridge.

 

About IDEA Bio-Medical Ltd.

IDEA Bio-Medical a company specializing in automated microscopy and image analysis for the life science researchers.  It was founded in 2007 through a partnership between YEDA (the Weizmann Institute’s commercialization arm) and IDEA Machine Development (an innovation hub).  IDEA’s products, The Hermes imaging system and Athena image analysis SW, have contributed to over 100 scientific publications in peer-reviewed magazines, globally supporting high impact science.

IDEA Bio-Medical currently focuses on empowering zebrafish researchers, specifically, to provide them with a reliable, robust solution for automated and unbiased Zebrafish image analysis by applying company’s knowledge and expertise.

To this end, IDEA developed a novel deep learning-based image analysis software for in vivo zebrafish experiments. The software automatically detects zebrafish contour and their internal organs in brightfield with no required user input. The anatomy identified is coupled to fluorescence channels to permit anatomy-specific study of fluorescence changes. It is an affordable, user-friendly system designed specifically for reliable, automated zebrafish image-based analysis.

The software is available as a stand-alone product and accepts microscopy images in multiple image formats, including proprietary ones. It is suited for researchers who only image and analyze a handful of fish per week, as well as researchers imaging hundreds and thousands of fish in multi-well plates for large scale screens. IDEA Bio-Medical is offering a novel pay-per-use model to access the software to enable flexible access.  So, all researchers using manual microscopes or automated systems from other vendors, can readily use IDEA’s Zfish software to extract quantitative, meaningful information from their Zebrafish images when they need it.

Reach out to IDEA Bio-Medical on our website's contact form, and more information can be found on the zebrafish analysis software product page.

 

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