Protein Analysis - Characterizing Cancer-Fighting Lipopeptides | Sample Preparation
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  June 13, 2019  
  Protein Analysis  
  The latest Protein Analysis news from across the AZoNetwork  
 

#ALT# Stable Isotope-Labeled Protein Standards for Quantification Using MS

Mass spectrometry (MS) is considered the preferred technique for protein analysis in the field of proteomics. One strategy for accurate quantification is to spike in heavy isotope-labeled standards corresponding to the proteins of interest. QPrESTs are heavy isotope-labeled forms of the unlabeled (light) protein epitope signature tags or PrESTs utilized as antigens for producing antibodies. Their unique sequence design makes them perfect for MS-based quantification in complex samples.

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    KeysightCharacterizing Cancer-Fighting Lipopeptides | Sample Preparation
 
Lipopeptides are cyclic structures produced by Bacillus and Pseudomonas species. They are hydrophilic peptides, usually between 7 and 10 amino acids long, linked to a hydrophobic fatty acid structure. They are part of a class of compounds that have been shown to have medicinal properties. This was shown by the ability of bacillus to successfully compete against plant and fungal pathogens in an antibiotic fashion. And also, by their ability to stimulate the defensive capabilities of athe plant.
 
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   Researchers reveal underlying mechanism that protects genetic transcripts in cellsResearchers reveal underlying mechanism that protects genetic transcripts in cells
 
The cell contains transcripts of the genetic material, which migrate from the cell nucleus to another part of the cell. This movement protects the genetic transcripts from the recruitment of "spliceosomes".
 
 Structural Motifs in Protein Biology
 
Structural Motifs in Protein BiologyAll proteins are made of basic secondary structure units, either α-helix or β-sheets, determined by hydrogen bonding between the amino acids within a peptide chain. On a larger scale, structures are formed by the combination of these secondary structures, and these can form supersecondary structures known as “motifs”.
 
 
 Why are Host Cell Proteins (HCPs) Dangerous in Biopharmaceuticals?
 
Why are Host Cell Proteins (HCPs) Dangerous in Biopharmaceuticals?The biopharmaceutical industry develops drugs that are based on recombinant proteins expressed in different host systems, such as bacteria, yeast, fungi, plant cells, etc. In these cases, the expressed proteins are subjected to several rounds of purification to ensure that the host cell proteins do not contaminate the resulting proteins of interest.
 
 
 Protein Crystallography Common Problems, Tips, and Advice
 
Protein Crystallography Common Problems, Tips, and AdviceFor most proteins that have had their structure determined, this has been done using protein crystallography. When the macromolecular structure of a protein is determined in this way, it is firstly crystalized by supersaturation, which causes each protein molecule to stack with its neighbors in a repeating pattern. The sample then undergoes x-ray crystallography, which is used to examine the structure of various crystal structures.