Protein Analysis - Enabling Ultrasensitive Protein Quantification With the Quanterix Homebrew Kit
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  The latest Protein Analysis news from across News-Medical  

#ALT# Protein Quantification Using Validated Stable Isotope-Labeled Standards

For absolute quantification of a protein, it is important to use a proteotypic peptide that ionizes well and gives a good signal. It's also important that the peptide is quantotypic.

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    KeysightEnabling Ultrasensitive Protein Quantification With the Quanterix Homebrew Kit
The Single Molecule Array (Simoa*) technology enables the detection and quantification of biomakers previously difficult or impossile to measure, opening up new applications to address significant unmet needs in life science research.
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   An Overview of Caspase ActivityAn Overview of Caspase Activity
Caspases are proteases: enzymes that catalyze the breakdown of proteins and are part of the cysteine–aspartic acid protease family. They are vital to the mechanism of programmed cell death in animals (apoptosis, pyroptosis, and necroptosis), and also play a fundamental role in inflammation. Due to their involvement in regulating cell death and inflammation, caspase activity is crucial to maintaining homeostasis in the body.
 What is Hypoxia-Inducible Factor?
What is Hypoxia-Inducible Factor?Hypoxia-inducible factor is a type of transcription factor that responds to the changing level of oxygen supply to the cell. The discovery of how it functions in the body to allow cells to respond to low oxygen levels has added a profound layer of understanding to how the body evolved this crucial capability. Further studies have elucidated the role of hypoxia-inducible factors in illness, such as anemia, cancer, and neurological disorders.
 Single-Molecule Fluorescence for the Study of GPCRs – Conformational Dynamics
Single-Molecule Fluorescence for the Study of GPCRs – Conformational DynamicsA review, by Professors Quast and Margeat from the French National Centre for Scientific Research exploring, has addressed G protein-coupled receptor (GPCR) structural dynamics and the role of single-molecule fluorescence SMF methods in their interrogation.
 Extensive tumor protein analysis reveals potential new players in cancer
Extensive tumor protein analysis reveals potential new players in cancerLooking to gain a deeper understanding of cancer, a team led by researchers at Baylor College of Medicine conducted an extensive computational analysis of the similarities and differences in the entire set of proteins, called the proteome, of more than 500 cancers from five different tissue sites.
 What is Fluorescent Western Blotting?
What is Fluorescent Western Blotting?Fluorescent western blotting is a powerful protein detection technique that offers numerous benefits in comparison to alternative chemiluminescent or chromogenic detection systems. It produces less chemical waste, is faster, and is a reliable method of collecting quantitative data.