Protein Analysis - Characterizing Cancer-Fighting Lipopeptides | Sample Preparation
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  June 13, 2019  
  Protein Analysis  
  The latest Protein Analysis news from across the AZoNetwork  

#ALT# Stable Isotope-Labeled Protein Standards for Quantification Using MS

Mass spectrometry (MS) is considered the preferred technique for protein analysis in the field of proteomics. One strategy for accurate quantification is to spike in heavy isotope-labeled standards corresponding to the proteins of interest. QPrESTs are heavy isotope-labeled forms of the unlabeled (light) protein epitope signature tags or PrESTs utilized as antigens for producing antibodies. Their unique sequence design makes them perfect for MS-based quantification in complex samples.

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    KeysightCharacterizing Cancer-Fighting Lipopeptides | Sample Preparation
Lipopeptides are cyclic structures produced by Bacillus and Pseudomonas species. They are hydrophilic peptides, usually between 7 and 10 amino acids long, linked to a hydrophobic fatty acid structure. They are part of a class of compounds that have been shown to have medicinal properties. This was shown by the ability of bacillus to successfully compete against plant and fungal pathogens in an antibiotic fashion. And also, by their ability to stimulate the defensive capabilities of athe plant.
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 Structural Motifs in Protein Biology
Structural Motifs in Protein BiologyAll proteins are made of basic secondary structure units, either α-helix or β-sheets, determined by hydrogen bonding between the amino acids within a peptide chain. On a larger scale, structures are formed by the combination of these secondary structures, and these can form supersecondary structures known as “motifs”.
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 Protein Crystallography Common Problems, Tips, and Advice
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