Fluorescence - New fluorescent probe specifically labels microglia cells
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  June 21, 2019  
  Fluorescence  
  The latest fluorescence news from NewsMedical  
 Plasmon-enhanced Fluorescence SpectroscopyPlasmon-enhanced Fluorescence Spectroscopy
 
Plasmon-enhanced fluorescence spectroscopy is a useful tool for imaging cells and other samples on a single-molecule level. This method poses several advantages over conventional fluorescence microscopy.
 
   New fluorescent probe specifically labels microglia cellsNew fluorescent probe specifically labels microglia cells
 
Part of the immune system in the brain is made up of so-called microglia cells. Korean and Singaporean researchers have now developed a fluorescent probe that specifically labels this type of macrophage. The cells were visualized in cell culture and in the live brains of rodents.
 
   Multiplex Approach to Fluorescent Western BlottingMultiplex Approach to Fluorescent Western Blotting
 
The quantitative nature of fluorescent tests compared to those which are based on the use of enzymes, like HRP, allows for more precise and simplified normalization, using an internal control such as a housekeeping gene.
 
 

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 Fluorescence Quenching
 
Fluorescence QuenchingWhen a molecule absorbs light, electrons in its constituent atoms become excited and are promoted to a higher energy level. When electrons in this excited state lose energy and return to the ground state, they release this energy in the form of heat or radiation. Light emitted during this process is known as fluorescence, and the molecules that show this activity are called fluorophores.
 
 
 Avoiding Cell Death in Fluorescence Microscopy
 
Avoiding Cell Death in Fluorescence MicroscopyFluorescence microscopy is a common laboratory tool used to investigate the physiology of different cells. Specific wavelengths of light are shone onto cells in a sample, where the light is absorbed by molecules called fluorophores.
 
 
 Advances in Fluorescence Microscopy
 
Advances in Fluorescence MicroscopyIn fluorescence microscopy, a specimen emits energy when activated by light of a certain wavelength. Some substances such as chlorophyll and some minerals will do this naturally, while others will need the help of other chemical triggers.