A Beginner's Guide to ChIP Webinar
Review this powerful technique, get advice on selecting antibodies, and learn troubleshooting techniques with our Epigenetic specialist
ChIP is a very powerful technique that allows for the analysis of protein localization on DNA regions in the genome. Selective enrichment of a chromatin fraction using antibodies allows for the isolation of DNA regions and interacting proteins.
About the Presenter
David Grotsky received his PhD in Molecular Genetics and Genomics from Washington University in St. Louis. His PhD thesis focused on studying the regulation of DNA repair factors and how they impact genomic stability and proliferation in tumor cells.
David is currently the Epigenetics Specialist on Abcam’s Scientific Support Team.
- What is ChIP?
- What is chromatin?
- Different types of ChIP
- ChIP protocol: chromatin preparation, sonication, immunoprecipitation, cross-linking reversion and DNA purification, DNA analysis, needed controls