Immunofluorescent Array Tomography Webinar
An introduction to immunofluorescent array tomography, with a special user's perspective
Review immunofluorescent array tomography- a method for studying synapses in the brain- in detail and see how it can be applied to other subcellular structures, such as mitochondria.
About the Presenters
Kristina D. Micheva
Kristina D. Micheva is a Senior Research Scientist in the Department of Molecular and Cellular Physiology at Stanford University, in California, USA. . Together with Dr. Stephen J Smith, Kristina is the co-inventor of array tomography. She has taught this imaging method at the Neurobiology Course at MBL in Woods Hole, Massachusetts for the past nine years.
Kristina is also a cofounder of Aratome, LLC, a company that is developing the commercial applications of array tomography. She is interested in synapse organization, function and plasticity, and the diversity of mammalian axons and synapses.
Thomas Misgeld is Professor for Neuronal Cell Biology at the Technical University Munich, Germany, and at the German Center for Neurodegenerative Diseases. He studied medicine in Munich before joining the lab of Jeff Lichtman in St. Louis and Boston as a post-doc.
Thomas has been a long-standing member of the teaching faculty of the Neurobiology Course at the Marine Biological Laboratory, Woods Hole, where he was introduced to the Array Tomography technique by Kristina Micheva and Stephen Smith.
- Introduction to array tomography methods
- Choosing antibodies and troubleshooting
- Investigating the protein composition of individual synapses
- Further applications: Studying organelle diversity