The manual preparation of sequencing libraries can be a labor-intensive, time consuming process that poses a hindrance in many laboratories. Manual processes are also associated with an increased risk of inconsistency and human error. To benefit from Illumina’s powerful next generation sequencing (NGS) technology, efficient and reliable automation solutions are essential for whole transcriptome library construction.
The Biomek® FXP Laboratory Automation Workstation (BFXP) combines every aspect of liquid handling required to automate NGS sample preparation in one compact system. The BFXP provides high-quality template libraries at the throughput needed to benefit from the high capacity of NGS, while offering the flexibility needed to meet the operator’s changing requirements.
Biomek® FXP Laboratory Automation Workstation
Now, scientists from Beckman Coulter have described the automation of Illumina’s TruSeq® Stranded Total RNA Sample Preparation kit on the BFXP for the construction of sequenceable whole transcriptome libraries. The kit removes ribosomal RNA (rRNA) while leaving mRNA and noncoding RNA behind, to enable whole-transcriptome sequencing through the generation of strand information. On the BFXP, up to 96 samples can be run simultaneously and the system can be set up with just a few clicks of the mouse and one-time pipetting of kit components into reservoirs. The ease and speed of reaction set-up is improved by the software and pipetting tools.
The BFXP has a user interface that enables the operator to select various options for the library construction set up including which steps are performed, how many samples are prepared, how incubations are handled and which adapter labware is used with which samples. Furthermore, a reagent calculator is provided which can tell the operator the volume required for each reagent as well as how they should be prepared.
The BFXP automation of the TruSeq Stranded Sample Preparation Kit enables the generation of up to 96 sequenceable libraries from as little as 0.1 μg of total RNA in just two days. The libraries constructed are of high quality, displaying consistent insert sizes, minimal adapter dimer, high alignment to the reference genome, and low numbers of ribosomal sequences, thereby enabling the transcriptome of interest to be analyzed in detail.
By using this automation solution to standardize NGS library construction, the risk of human error is reduced, which lowers reagent cost and frees up valuable time for researchers so they can proceed with other tasks in the laboratory.
For more information on this automation method click here.
About Beckman Coulter
Beckman Coulter develops, manufactures and markets products that simplify, automate and innovate complex biomedical tests. More than a quarter of a million Beckman Coulter instruments operate in laboratories around the world, supplying critical information for improving patient health and reducing the cost of care.
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