Forensic Science Ireland (FSI) has developed a method to extract commonly targeted drugs and drug metabolites from urine samples related to sexual assaults. Solid-phase extraction methods are used before performing screening analysis via LCMSMS. The sample eluent is allowed to evaporate completely.
As part of its validation process, FSI assessed the application of the Genevac EZ-2 personal evaporator as an optional approach (Figure 1). In traditional blowdown evaporation, gases like nitrogen are used. In contrast, Genevac’s EZ-2 system prevents the need for such gases but also reduces reformatting stages and sample handling tasks.
Figure 1. Genevac EZ-2 Evaporator.
Choice of Evaporation Procedure
Traditionally a nitrogen blowdown system is used for evaporation of the analytes. However this method causes deposition on the tube sides and, with a reconstitution volume of the only 200µl, all the analytes could not be resuspended. In order to realize maximum recovery, sequential evaporation in the MS analysis vial is required.
In the EZ-2 system, the solvent is boiled at low temperature under vacuum conditions. During evaporation, the samples are centrifuged at 500G, depositing the dried analyte at the bottom of the tube. Therefore, reconstitution is possible even in low volumes.
The experiments show that with the EZ-2 system, evaporation is simplified. Sample integrity is sustained with minimum time devoted to the evaporation process. Better sensitivity without cross-contamination is achieved, indicating the advantages of evaporation using the EZ-2 when compared to traditional evaporation techniques.
- Produced from materials authored by Paula Clarke1 and Alison Wake2 from: Forensic Science Ireland, Garda Headquarters, Dublin, Ireland
- Genevac Ltd, Ipswich, UK
About SP Scientific Products
SP Scientific is the synergistic collection of well-known, well-established, and highly regarded scientific equipment brands — VirTis, FTS Systems, Hotpack, Hull, Genevac, PennTech, and most recently i-Dositecno — joined to create one of the largest and most experienced companies in freeze-drying/lyophilization, production fill-finish lines, centrifugal evaporation and concentration, temperature control/thermal management, glassware washers and controlled environments.
More than a scientific equipment supplier, SP Scientific represents brands that distinguish themselves by thoroughly assist customers in matching equipment to particular application needs. Fortune 500, pharmaceutical, aerospace, automotive, medical device, diagnostic kit, and biotechnology companies — as well as government facilities, universities, and colleges, are among the organizations served on a daily basis.
SP Scientific is part of SP Industries, Inc., a leading designer, and manufacturer of state-of-the-art laboratory equipment, pharmaceutical manufacturing solutions, laboratory supplies and instruments, and specialty glassware. SP's products support research and production across divers end-user markets including pharmaceutical, scientific research, industrial, aeronautic, semiconductor, and healthcare.
SP's three flagship brands -- SP Scientific, SP Scienceware, and SP Ableware-- represent over 400 combined years of experience, quality, and innovation. Headquartered in Warminster, Pennsylvania, SP Industries has production facilities in the USA and Europe and offers a world-wide sales and service network with full product support including training and technical assistance.
Sponsored Content Policy: News-Medical.net publishes articles and related content that may be derived from sources where we have existing commercial relationships, provided such content adds value to the core editorial ethos of News-Medical.Net which is to educate and inform site visitors interested in medical research, science, medical devices and treatments.