Cultures Prepared by Department of Pathology and Laboratory Medicine, State Public Health Laboratory of Nevada
Investigation of infectious bacteria
- After inoculating water-based cultures with 12 infectious bacteria, they were incubated for 24 hours at 37°C before starting the analysis of headspace vapors using a GC/SAW electronic nose. The results revealed that two of the 12 cultures did not develop and were free from bacteria.
- Headspace vapor of cultures lacking bacteria showed 2 major nutrient analyte peaks and 3 small analyte peaks situated in the middle of the two nutrient peaks
- The growth of bacteria had a dynamic interaction with nutrient compounds and formed new analyte vapors that are specific to the bacteria itself. The combination of concentrations of new analytes and modified nutrient vapors formed VaporPrints® images specific to each type of the bacteria.
- E.Coli and E.Coli O157 created nearly identical VaporPrints®, but one analyte was identified to be specific to only O157
- In general, it is possible to recognize each type of bacteria by its characteristic VaporPrint® image
Hemophilus influenza and strep pneumonia
The results showed no bacterial growth for Hemophilus influenza and Strep pneumonia culture bottles.
Escherichia coli O157
E. coli (benign)
Comparison of E. coli
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