Using a GC/SAW Electronic Nose for Investigation of Infectious Bacteria

VaporPrint® of infectious bacteria cultures

Cultures Prepared by: Department of Pathology and Laboratory Medicine, State Public Health Laboratory of Nevada

Investigation of infectious bacteria

  • After inoculating water-based cultures with 12 infectious bacteria, they were incubated for 24 hours at 37°C before starting the analysis of headspace vapors using a GC/SAW electronic nose. The results revealed that two of the 12 cultures did not develop and were free from bacteria.
  • Headspace vapor of cultures lacking bacteria showed 2 major nutrient analyte peaks and 3 small analyte peaks situated in the middle of the two nutrient peaks
  • The growth of bacteria had a dynamic interaction with nutrient compounds and formed new analyte vapors that are specific to the bacteria itself. The combination of concentrations of new analytes and modified nutrient vapors formed VaporPrints® images specific to each type of the bacteria.
  • E.Coli and E.Coli O157 created nearly identical VaporPrints®, but one analyte was identified to be specific to only O157
  • In general, it is possible to recognize each type of bacteria by its characteristic VaporPrint® image

Hemophilus influenza and strep pneumonia

The results showed no bacterial growth for Hemophilus influenza and Strep pneumonia culture bottles.

Candida albicans

Shigella flexneri

Staphylococcus aureus

Pseudomonas aeruginosa

Salmonella enteritidis

Enterococcus faecalis

Klebsiella pneumonia

Escherichia coli O157

E. coli (benign)

Comparison of E. coli

Salmonella typhimurium

About Electronic Sensor Technology

Electronic Sensor Technology

Electronic Sensor Technology, Inc has developed and patented a breakthrough chemical vapor analysis process. This process applies gas chromatography calculations and technology toward a wide variety of industries, including Homeland Security, Life Sciences, Chemical and Petrochemical, Food & Beverage and Environmental.

How does gas chromatography work? With rapid, accurate analysis of chemical odors and vapors, this patented technology helps to provide real-time analysis for quick response solutions.

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Last updated: May 22, 2017 at 2:31 PM

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