A Guide to Identifying Histone Modifications

This article presents a cheat sheet to identify which histone modification marks which genomic element. Abcam have produced this guide for users so that they don’t have to continuously reference the literature to find these answers.

Histone modifications and where to find them

The following table lists the most common histone modifications and the genomic regions they mark, that is, where users can expect to find them in their ChIP/ChIPseq experiments. In order to provide a little more information, the article has also listed the most specific antibodies that are used to recognize those histone modifications.

Histone mark

Where to find it

Antibody

H3K4me1

Active enhancers

ab176877

H3K4me3

Promoters of active genes, bivalent domains

ab8580

H3K9ac

Active enhancers and promoters

ab177177

H3K27ac

Active enhancers and promoters

ab177178

H3K36me3

Transcribed regions

ab9050

H3K79me2

Transcribed regions

ab177184

H3K9me3

Heterochromatin, satellite repeats, gene-poor regions

ab176916

H3K27me3

Repressed promoters in gene-rich regions, developmental regulators, bivalent domains

ab192985

Gamma H2A.X

DNA double-strand breaks

ab81299

H3S10P

Whole chromosomes in mitosis

ab177218

H4K16ac

Reduced in cancer at repetitive sequences, active genes and enhancers in ESCs. Inhibits formation of high order chromatin structure.

ab109463

Green = generally associated with transcriptionally active regions; red = generally associated with transcriptionally repressed regions; grey = other functions.

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Last updated: Jun 10, 2019 at 3:52 AM

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