Real-Time Imaging of Amyloid Beta with Near Infrared Fluorescent (NIRF) Probes

Introduction

Near infrared fluorescent (NIRF) probes allow real-time imaging of amyloid beta (Aß) and are developed for both in vivo and in vitro imaging.

The deposition of Aß in the human brain has been recognized as the earliest pathological characteristic of Alzheimer’s disease. As a result, an increasing number of imaging probes have been developed in the past 10 years which have enabled the detection of Aß deposits in a non-invasive manner. These imaging probes mainly depend on the following techniques:

  • magnetic resonance imaging (MRI)
  • single-photon emission computed tomography (SPECT)
  • positron emission tomography (PET)

NIRF probes are safe, cost-effective, and provide a high resolution alternative technique for real-time imaging of insoluble and soluble Aß in vitro and in vivo.

The advantages of NIRF probes

  • Ideal for in vivo imaging Since emission wavelengths are in the NIR range, background fluorescence from brain tissue is minimized.
  • Detection of both insoluble and soluble Aß Abcam provides a complete range of probes that make it possible to detect both fibrils and Aß monomers, as shown in Figure 1.
  • Cell-permeable — All Abcam’s NIRF probes are capable of crossing the blood brain barrier (BBB), which enable the labeling of Aß in the brain.
  • Safe to use — All NIRF probes are non-radioactive, which prevents unwanted exposure to radiation.
  • Inexpensive — Data analysis is fast, the costs of the imaging instrument are also low, and highly skilled workers are not needed.

Figure 1.  NIRF probes binding to Aß monomers and fibrils.

Available NIRF probes

Product name

λexem (nm)

Aß species detected

References

CRANAD-58 (ab146926)

630/672

Aß42 and Aß40 monomers, Aß40 fibrils

1, 2

MCAAD-3 (ab216983)

596/685

Aß42 fibrils

1, 3

CRANAD-2 (ab141775)

640/715

Aß40 fibrils

1, 4

 

References

  1. Xu, M.-M., Ren, W.-M., Tang, X.-C., Hu, Y.-H. & Zhang, H.-Y. Advances in development of fluorescent probes for detecting amyloid-β aggregates. Nat. Publ. Gr. 37, 719–730 (2016).
  2. Chongzhao, R. et al. Design, synthesis, and testing of difluoroboron-derivatized curcumins as near-infrared probes for in vivo detection of amyloid-ß deposits. J. Am. Chem. Soc. 131, 15257–15261 (2009).
  3. Fu, H., Cui, M., Tu, P., Pan, Z. & Liu, B. Evaluation of molecules based on the electron donor-acceptor architecture as near-infrared β-amyloidal-targeting probes. Chem. Commun. (Camb). 50, 11875–8 (2014).
  4. Chongzhao, R. et al. Design, synthesis, and testing of difluoroboron-derivatized curcumins as near-infrared probes for in vivo detection of amyloid-ß deposits. J. Am. Chem. Soc. 131, 15257–15261 (2009).

About Abcam

Abcam is a global life sciences company providing highly validated antibodies and other binders and assays to the research and clinical communities to help advance the understanding of biology and causes of disease.

Abcam’s mission is to serve life scientists to help them achieve their mission faster by listening to their needs, continuously innovating and improving and by giving them the tools, data and experience they want. Abcam’s ambition is to become the most influential life science company for researchers worldwide.

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Last updated: Jul 14, 2018 at 6:34 PM

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