Label-Free 3D Live Cell Imaging Revolutionizes Stem Cell Research

Research into stem cells comprises arguably the greatest obstacle to current study in biological sciences. However, knowing more about them could be the key to evolving cures to aging, cancer and degenerative disease, such as Alzheimer’s disease or organ-related failures like heart disease.

3D Cell Explorer Advances Stem Cell Research

Nanolive has brought out the 3D Cell Explorer for live cell imaging under the microscope with unparalleled resolution, flexibility and contrast while using a label-free approach. At the same time, temporal resolution is also superb. And finally, it offers a 3D visualization.

Here an overview of some stunning stem cell images are shown to present the scope of this platform.

Long Term Live Imaging of Mouse Embryonic Stem Cells for 15 hours (click here for 3D view)

Long Term Live Imaging of Mouse Embryonic Stem Cells for 34 hours!

High-Frequency, Long-Term Live Imaging

The reason for the difficulty with stem cell research is the inability to visualize fine cellular processes in a dynamic fashion over the long term. The great appeal of the 3D Cell Explorer is in the lack of any phototoxic effects on the cells imaged, as the energy it leaves in the sample is a hundred times less, at about 0.2 nW/µm2, compared to 1nW/µm2 with light sheet microscopes, which are the gold standard in this field.

It also offers a resolution of 195 nm which exceeds that offered by standard confocal microscopy. Thus the 3D Cell Explorer allows even sensitive material to be imaged at high resolution and high frequency, including stem cells.

Also, it allows clear examination of cell organelles as well as tiny or very mobile structures within the cell, including mitochondria, droplets of lipids, filopodia, axons and dendrites, as well as the nucleus and nucleoli, with respect to the specific dynamic processes occurring within them.

Moreover, this unique microscope allows live imaging to be performed at the highest possible speed of image acquisition, of 1 image/1.7 seconds. This is much higher than that achieved by any other microscope, and is a drastically new development in stem cell live imaging.

Another big advantage is the reduction in the cost of making live cell imaging movies, because of the use of advanced holotomographic technology which costs only a fraction of a standard confocal microscope.

Long Term Live Imaging of Mouse Embryonic Stem Cells for 9,5 hours! Part 1

Long Term Live Imaging of Mouse Embryonic Stem Cells for 48 hours! Part 2

To find out more about how the 3D Cell Explorer works and what it means for the long-term live imaging of stem cells, see: https://nanolive.ch/stemcell-webinar/.

About Nanolive SA

Nanolive SA are scientists, working for scientists.

Their belief is that each and every Biologist, Researcher and Physician should be able to explore and interact instantly with living cells without damaging them.

Nanolive want to support the study of how living cells and bacteria work, evolve and react, thus building a solid base for new drugs and therapies, in order to enable breakthrough researches.

This is the reason why they have developed the 3D Cell Explorer.


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Last updated: Mar 12, 2019 at 4:00 AM

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