Antibody Arrays for Multiplex Protein Analysis

Antibody arrays from Abcam can are used for multiplex protein analysis and are available for human other model species.

Antibody arrays provide the following advantages:

  • Analysis is simple and can be run using a laser slide scanner
  • Up to 40 different proteins can be analyzed in each antibody array, requiring as little as 50 µL of sample
  • The arrays can be run in parallel, allowing full profiling of the 400+ human cytokines, 200 mouse cytokines, as well as the cytokines of other model species.
  • As with ELISA experiments, the arrays are on glass slides and use sandwich antibody pairs

Specialized quantitative multiplex cytokine and antibody arrays can be provided for more than 400 human proteins and 200 mouse proteins. Abcam can also provide arrays for other model species including the proteins found in non-human primates, pigs, cows, dogs, horses, cats and rats.

To assist with the comparison of quantitative antibody arrays provided for different species, Abcam provides information on the different cytokines and proteins that can be supplied for each array.

Running an array is fast and simple. Arrays can be run in a single day and if the process has been optimized, a runtime of four hours can be achieved. Quantitative analysis of arrays can be achieved using the majority of gene microarray scanners or laser glass slide scanners. The fluorophore dye used has the same excitation and relaxation/emission wavelengths as cyanine Cy3.

To achieve an even greater throughput, four slides can be slotted into a tray that matches a standard microplate (available on request). This facilitates automated processing using liquid handling.

Array Format

Each of the glass sides has 16 identical antibody array spots and also has a 16-well gasket, which allows different samples to be analyzed on the same array. Antibodies are spotted four times on each array.

Antibody Specificity

Quantitative cytokine and antibody arrays from Abcam have been stringently tested to make sure no false positives are produced. These tests have been carried out with protein target mixtures (at 10 ng/mL) on individual detection antibodies.

​​​​​​​​​​​​​​​​​​Dynamic Range and Sensitivity

The detection sensitivity of Abcam’s quantitative cytokine and antibody arrays have been tested against corresponding ELISA kits, and it has been observed that there is a similar level of detection between the two methods with a greater dynamic range for antibody arrays.

The technology used in Abcam’s arrays has been proven to work over 200 times in the literature.

Calibration Curve

To provide reproducible and accurate results, eight of the arrays are run with a mixture of protein standards to provide a calibration curve. The eight different calibration curve arrays can be run over different slides, as long as two points on each slide include a standard to account for variations.

This facilitates the measurement of 8 experimental samples using one slide, 22 samples with two slides or 36 samples with three slides.

GAL files can be downloaded which provide an array map for fluorescent densitometry for Abcam’s quantitative cytokine and antibody arrays.

​Semi-Quantitative Membrane Arrays Require No Laser Scanner

If a suitable scanner is not available or accessible (for example through a genomics research institute) then semi-quantitative membrane arrays can be used. Membrane arrays can be analyzed using the same set-up as western blot methods. Abcam provides information on which analytes can be measured using semi-quantitative membrane arrays.

About Abcam

Abcam is a global life sciences company providing highly validated antibodies and other binders and assays to the research and clinical communities to help advance the understanding of biology and causes of disease.

Abcam’s mission is to serve life scientists to help them achieve their mission faster by listening to their needs, continuously innovating and improving and by giving them the tools, data and experience they want. Abcam’s ambition is to become the most influential life science company for researchers worldwide.

Sponsored Content Policy: publishes articles and related content that may be derived from sources where we have existing commercial relationships, provided such content adds value to the core editorial ethos of News-Medical.Net which is to educate and inform site visitors interested in medical research, science, medical devices and treatments.

Last updated: Apr 1, 2019 at 6:05 AM


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