Fast and Simple, Single-Step Antibody Analysis

Abcam’s SimpleStep ELISA® kits make analysis simple. The kits use a semi-homogenous method, which allows the antibodies and samples to be added in a single step. This method also results in a better sensitivity than sequential sandwich ELISA formats.

The antibody-sample sandwich forms quickly following mixing before it binds to the plate. The speed of this interaction means that the sample/antibody mixture only needs one hour of incubation.

SimpleStep ELISA Kit

The Simple Step method can also be more sensitive. As shown in Figure 1, the CXCL16 SimpleStep ELISA kit has better sensitivity than a conventional, heterogeneous ELISA kit method, despite this method requiring two additional steps (three in total).  

Standard comparison between Human CXCL16 SimpleStep ELISA kit (ab187397)​ and a conventional ELISA kit.

Figure 1. Standard comparison between Human CXCL16 SimpleStep ELISA kit (ab187397) and a conventional ELISA kit.

Similarly, increased sensitivity was seen for Enolase 1 (ENO1) (Figure 2).

Standard curve comparison between Human ENO1 SimpleStep ELISA kit (ab181417) and a standard sequential ELISA kit.

Figure 2. Standard curve comparison between Human ENO1 SimpleStep ELISA kit (ab181417) and a standard sequential ELISA kit.

About Abcam

Abcam is a global life sciences company providing highly validated antibodies and other binders and assays to the research and clinical communities to help advance the understanding of biology and causes of disease.

Abcam’s mission is to serve life scientists to help them achieve their mission faster by listening to their needs, continuously innovating and improving and by giving them the tools, data and experience they want. Abcam’s ambition is to become the most influential life science company for researchers worldwide.


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Last updated: Apr 1, 2019 at 5:39 AM

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