The T-cell receptor (TCR) cannot signal on its own. Rather, is constitutively associated with CD3 coreceptors, which contain intracellular signaling apparatus comprised of CD3εγ and CD3εδ heterodimers, as well as the CD3ζζ homodimer (Wucherpfennig KW, et al., 2010; Kuhns MS, et al., 2012). In the past few decades, CD3 has become the focus of pharmaceutical studies.
Allograft rejection and autoimmune diseases have been treated using direct inhibition of the CD3 protein. In 1979, P. Kung and G. Goldstein (Kung P, et al., 1979) produced OKT3, the first mouse monoclonal antibody specific for human CD3. This was also the first monoclonal antibody to be used in clinical practice, in the field of transplantation.
In the last couple of decades, CD3 targeting has been used as an approach for bridging cytotoxic T cells to target cells in bi-specific antibody design. A successful example is Amgen’s Blincyto®, or blinatumomab, which is a bi-specific antibody that simultaneously targets CD3 and CD19. By doing so, it directs the CD3-expressing T cells to CD19-expressing lymphoma cells (Nagorsen D, et al., 2012). It would be possible to extend the potential of CD3-specific antibody therapy to promote tolerance, an idea that has been proven to work for autoimmune diseases, transplantation and other autoimmune diseases.
Image credit: ACRO Biosystems
ACROBiosystems offers a comprehensive panel of CD3 proteins, such as the unique Biotinylated Human CD3 epsilon (Cat. No. CDE-H8223) and the Biotinylated Human CD3E and CD3D heterodimer protein (Cat. No. CDD-H82W0). It is also the only supplier of Mouse CD3 epsilon protein (Cat. No. CDE-M5256).
CD3 Product Highlight
Immobilized Anti-Human CD3 Mab, mouse IgG1 (Clone # SP34-2) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD3E, His Tag (Cat. No. CDE-H8223) with a linear range of 0.05-2 ng/mL. Image credit: ACRO Biosystems
Immobilized Biotinylated Human CD3E and CD3D (Cat. No. CDD-H82W0) can bind OKT3 with a linear range of 2-39 ng/mL. The biotinylated CD3E and CD3D heterodimers are coated at 1 ug/mL (100 μL/well) on streptavidin-coated plates. Image credit: ACRO Biosystems
CD3 Product List
| Molecule |
Cat. No. |
Species |
Structure |
Purity |
Features |
| CD3 epsilon (Biotin-labeled) |
CDE-H8223 |
 |
 |
 |
 |
| CD3 epsilon |
CDE-H5223 |
|
 |
|
|
| CD3 epsilon |
CDE-H5256 |
|
 |
|
|
| CD3 epsilon |
CDE-H5286 |
|
 |
|
|
| CD3 epsilon |
CDE-M5256 |
 |
 |
|
|
| CD3 epsilon |
CDE-C5226 |
 |
 |
|
|
| CD3 epsilon |
CDE-C5254 |
|
 |
|
|
| CD3 delta |
CDD-H5224 |
|
 |
|
|
| CD3 delta |
CDD-C52H8 |
|
 |
|
|
| CD3E & CD3D (Biotin-labeled) |
CDD-H82W0 |
|
 |
|
|
| CD3E & CD3D |
CDD-H52W4 |
|
 |
|
|
| CD3E & CD3D |
CDD-H52W0 |
|
 |
|
|
| CD3E & CD3D |
CDD-C52W4 |
|
 |
|
|
| CD3E & CD3G |
CDG-H52W9 |
|
 |
|
|
Related Products
| Molecule |
Cat. No. |
Species |
Structure |
Purity |
Features |
| CD4 (Biotin-labeled) |
CD4-H82E8 |
|
 |
|
|
| CD4 (Biotin-labeled) |
CD4-H82F3 |
|
 |
|
|
| CD4 |
LE3-H5228 |
|
 |
|
|
| CD4 |
CD4-H5259 |
|
 |
|
|
| CD4 |
CD4-C52H7 |
 |
 |
|
|
| CD4 |
CD4-C5256 |
|
 |
|
|
References
- Kung, P., et al., 1979, Science 206: 347–349.
- Wucherpfennig KW, et al., 2010, Cold Spring Harb Perspect Biol 2: a005140.
- Kuhns MS, et al., 2012, Immunol Rev 250: 120–143.
- Nagorsen D., et al., 2012, Pharmacology & Therapeutics 136: 334–342.
ACROBiosystems
ACROBiosystems is an internationally recognized manufacturer of recombinant proteins committed to supporting cancer immunotherapy. We specialize in mammalian cell-based recombinant protein production and process development.
Our goal is to support professionals from pharmaceutical companies, CROs and research institutes who are working on the cancer immunotherapy area by providing high-quality proteins, antibodies and assay kits.
We have multiple offices and branches in North America, Europe, and Asia, and we are proud of serving customers from over 50 countries.
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