Protein Electrophoresis Using SDS-PAGE Gels

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Abcam’s Optiblot SDS-PAGE gels are an upgrade to the conventional gels, both in their improved performance and ease of use.

Currently, gels are available in a 12 or 17 well format with 10 gels per pack. Cassette sizes are compatible with most common tank systems, including XCell and Mini-PROTEAN gel tanks.

Optiblot gels should be used with Optiblot buffers to maximize running conditions - buffer recipes are provided with gels or can be downloaded.

Optiblot Gel Products

10 x 10 cm gels:

Recommended for XCell gel tanks.

Product name Gel type Product code
Optiblot SDS Gel 8% - 12 well Single concentration ab139591
Optiblot SDS Gel 10% - 12 well Single concentration ab119202
Optiblot SDS Gel 12% - 12 well Single concentration ab119203
Optiblot SDS Gel 16% - 12 well Single concentration ab139592
Optiblot SDS Gel 4–8% - 12 well Gradient ab119204
Optiblot SDS Gel 4–12% - 12 well Gradient ab139593
Optiblot SDS Gel 4–20% - 12 well Gradient ab119205
Optiblot SDS Gel 8% - 17 well Single concentration ab139597
Optiblot SDS Gel 10% - 17 well Single concentration ab139598
Optiblot SDS Gel 12% - 17 well Single concentration ab139599
Optiblot SDS Gel 16% - 17 well Single concentration ab139600
Optiblot SDS Gel 4–8% - 17 well Gradient ab139601
Optiblot SDS Gel 4–12% - 17 well Gradient ab139602
Optiblot SDS Gel 4–20% - 17 well Gradient ab139603

8 x 10 cm gels:

Recommended for Mini-PROTEAN gel tanks.

Product name Gel type Product code
Optiblot SDS Gel 8% - 12 well Single concentration ab139594
Optiblot SDS Gel 10% - 12 well Single concentration ab119206
Optiblot SDS Gel 12% - 12 well Single concentration ab119207
Optiblot SDS Gel 16% - 12 well Single concentration ab139595
Optiblot SDS Gel 4–8% - 12 well Gradient ab119208
Optiblot SDS Gel 4–12% - 12 well Gradient ab139596
Optiblot SDS Gel 4–20% - 12 well Gradient ab119209
Optiblot SDS Gel 8% - 17 well Single concentration ab139604
Optiblot SDS Gel 10% - 17 well Single concentration ab139605
Optiblot SDS Gel 12% - 17 well Single concentration ab139606
Optiblot SDS Gel 16% - 17 well Single concentration ab139607
Optiblot SDS Gel 4–8% - 17 well Gradient ab139608
Optiblot SDS Gel 4–12% - 17 well Gradient ab139609
Optiblot SDS Gel 4–20% - 17 well Gradient ab139610

Anchor Gel Cassette Compatability with Common Tanks

Tank system 10 x 10 cm Optiblot gel 8 x 10 cm Optiblot gel
Bio-Rad Mini-PROTEAN No Yes
Invitrogen XCell II Yes No
Invitrogen XCell Surelock Yes No
Thermo Scientific Owl P82 Yes No
CBS Scientific Quadra Combo Yes Yes

Running Conditions

. .
Voltage 180 V
Start current 90 mA/gel
End current 40 mA/gel
Run time 40–55 min

About Abcam

Abcam is a global life sciences company providing highly validated antibodies and other binders and assays to the research and clinical communities to help advance the understanding of biology and causes of disease.

Abcam’s mission is to serve life scientists to help them achieve their mission faster by listening to their needs, continuously innovating and improving and by giving them the tools, data and experience they want. Abcam’s ambition is to become the most influential life science company for researchers worldwide.


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Last updated: Apr 1, 2019 at 6:39 AM

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