Finding a Secure and Consistent Supply of Polyclonal Antibodies

As the selectivity and the stable supply of polyclonal antibodies becomes an area of more concern, Abcam can provide solutions to allow researchers to continue to undertake accurate and consistent experiments.

Polyclonal Antibodies

Many polyclonal antibodies supplied by many life science suppliers are soon to be become unavailable, which could result in slowing down their research. However, to solve this problem, Abcam provides several excellent alternative antibodies.

For example, the most cited antibody anti-ERK1, was discontinued in a competitors range in 2017. In addition to the fact that this antibody is now difficult to source it also exhibits cross-reactions with non-target proteins (Figure 1), which makes data interpretation hard to carry out.

Western blot results for another supplier

Figure 1. Western blot results for another supplier's top-cited anti-ERK1 antibody. Predicted band size: 43 kDa. Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg). Lanes 2, 4 and 6: ERK1 knockout HAP1 cell lysate (20 µg). Lanes 1 and 2: green signal from top-cited anti-ERK1 antibody. Lanes 3 and 4: red signal from loading control (ab8245). Lanes 5 and 6: merged (red and green) signal.

To replace the anti-ERK1 antibody Abcam can provide an anti-ERK1 RabMAb® rabbit monoclonal antibody that has been knockout validated using a CRISPR method. The anti-ERK1 RabMAb® rabbit monoclonal gives consistent results between batches, shows highly selective binding (Figure 2) and also ensures a secure supply.

Left: western blot results from our anti-ERK1 antibody (ab32537). Predicted band size: 43 kDa. Lanes 1, 3 and 5: wild-type HAP1 cell lysate (20 µg). Lanes 2, 4 and 6: ERK1 knockout HAP1 cell lysate (20 µg). Lanes 1 and 2: green signal from target (ab32537) observed at 42 kDa. Lanes 3 and 4: red signal from loading control (ab8226) observed at 42 kDa. Lanes 5 and 6: merged (red and green) signal. Right: immunocytochemistry data from our anti-ERK1 antibody (ab32537) in wild-type HAP1 cells (top panel) and ERK1 knockout HAP1 cells (bottom panel). DNA labeled in blue with DAPI.

Figure 2. Left: western blot results from our anti-ERK1 antibody (ab32537). Predicted band size: 43 kDa. Lanes 1, 3 and 5: wild-type HAP1 cell lysate (20 µg). Lanes 2, 4 and 6: ERK1 knockout HAP1 cell lysate (20 µg). Lanes 1 and 2: green signal from target (ab32537) observed at 42 kDa. Lanes 3 and 4: red signal from loading control (ab8226) observed at 42 kDa. Lanes 5 and 6: merged (red and green) signal. Right: immunocytochemistry data from our anti-ERK1 antibody (ab32537) in wild-type HAP1 cells (top panel) and ERK1 knockout HAP1 cells (bottom panel). DNA labeled in blue with DAPI.

If you want to ensure the supply of your antibodies and accuracy of your research, Abcam can help you find replacement options for those.

About Abcam

Abcam is a global life sciences company providing highly validated antibodies and other binders and assays to the research and clinical communities to help advance the understanding of biology and causes of disease.

Abcam’s mission is to serve life scientists to help them achieve their mission faster by listening to their needs, continuously innovating and improving and by giving them the tools, data and experience they want. Abcam’s ambition is to become the most influential life science company for researchers worldwide.


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Last updated: Apr 23, 2019 at 9:58 AM

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