The High Affinity of Rabbit Monoclonal Antibodies

The affinity between an antibody and its antigen is usually described using the equilibrium dissociation constant (KD) – which is the ratio of koff/kon. Using this method a low KD value indicates a high affinity relationship, i.e. stronger binding.1

While most therapeutic monoclonal antibodies have a KD value in the nanomolar range (KD = 10-9 M), rabbit monoclonals steadily demonstrate higher affinity, with the KD values which can often reach the picomolar level (KD = 10-12 M), eliminating the requirement for further affinity maturation.2

For researchers carrying out experiments a high affinity, i.e. a low KD value, means that they can carry out research under stricter conditions without compromising their signal strength. Ultimately, this means that they can collect the cleanest data at the best specificity possible.

Affinity Comparison by KD Value for Antibodies Generated with RabMAb® Ttechnology vs. Popular Therapeutic Antibodies

RabMAb KD (M) Therapeutic MAb KD (M)
ER 1.28 x 10-12 Herceptin 5.0 x 10-9
ID1 2.82 x 10-12 Rituxan 8.0 x 10-9
C29 9.57 x 10-11 Synagis 1.0 x 10-9
TNF-alpha 1.25 x 10-11 Remicade 2.0 x 10-10
IL-1-beta 1.99 x 10-10 Avastin 5.0 x 10-10

Affinity Meter: RabMAb Primary Antibodies vs. Typical Monoclonal Antibodies

Antibody affinity comparison by KD value for RabMAb primary antibodies vs. typical monoclonal antibodies. Measurement based on KD measurement analysis of over 850 RabMAb primaries. RabMAb primary antibodies display antibody affinity level in the picomolar range compared to typical MAbs which are in the nano molecular range.

Antibody affinity comparison by KD value for RabMAb primary antibodies vs. typical monoclonal antibodies. Measurement based on KD measurement analysis of over 850 RabMAb primaries. RabMAb primary antibodies display antibody affinity level in the picomolar range compared to typical MAbs which are in the nano molecular range.

References

  1. Applications And Engineering Of Monoclonal Antibodies. David J. King, 2007.
  2. Anti-peptide antibody screening: Selection of high affinity monoclonal reagents by a refined surface plasmon resonance technique. Pope ME, [J Immunol Methods. 2009 Feb 28;341(1-2):86-96. doi: 10.1016/j.jim.2008.11.004. Epub 2008 Nov 28]

About Abcam

Abcam is a global life sciences company providing highly validated antibodies and other binders and assays to the research and clinical communities to help advance the understanding of biology and causes of disease.

Abcam’s mission is to serve life scientists to help them achieve their mission faster by listening to their needs, continuously innovating and improving and by giving them the tools, data and experience they want. Abcam’s ambition is to become the most influential life science company for researchers worldwide.


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Last updated: May 2, 2019 at 9:33 AM

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