The affinity between an antibody and its antigen is usually described using the equilibrium dissociation constant (KD) – which is the ratio of koff/kon. Using this method a low KD value indicates a high affinity relationship, i.e. stronger binding.1
While most therapeutic monoclonal antibodies have a KD value in the nanomolar range (KD = 10-9 M), rabbit monoclonals steadily demonstrate higher affinity, with the KD values which can often reach the picomolar level (KD = 10-12 M), eliminating the requirement for further affinity maturation.2
For researchers carrying out experiments a high affinity, i.e. a low KD value, means that they can carry out research under stricter conditions without compromising their signal strength. Ultimately, this means that they can collect the cleanest data at the best specificity possible.
Affinity Comparison by KD Value for Antibodies Generated with RabMAb® Ttechnology vs. Popular Therapeutic Antibodies
||1.28 x 10-12
||5.0 x 10-9
||2.82 x 10-12
||8.0 x 10-9
||9.57 x 10-11
||1.0 x 10-9
||1.25 x 10-11
||2.0 x 10-10
||1.99 x 10-10
||5.0 x 10-10
Affinity Meter: RabMAb Primary Antibodies vs. Typical Monoclonal Antibodies
Antibody affinity comparison by KD value for RabMAb primary antibodies vs. typical monoclonal antibodies. Measurement based on KD measurement analysis of over 850 RabMAb primaries. RabMAb primary antibodies display antibody affinity level in the picomolar range compared to typical MAbs which are in the nano molecular range.
- Applications And Engineering Of Monoclonal Antibodies. David J. King, 2007.
- Anti-peptide antibody screening: Selection of high affinity monoclonal reagents by a refined surface plasmon resonance technique. Pope ME, [J Immunol Methods. 2009 Feb 28;341(1-2):86-96. doi: 10.1016/j.jim.2008.11.004. Epub 2008 Nov 28]
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