Secondary Antibodies: HRP-polymer Type

The use of HRP-polymer secondary antibodies is recommended to save time when completing an IHC experiment, to increase sensitivity and to avoid background due to endogenous biotin.

Micropolymer Technology

Micropolymer technology is used by HRP-polymer secondary antibodies to form smaller detection complexes that allow better tissue penetration and sensitivity. Also, HRP-polymer secondaries bind more horseradish peroxidase than standard HRP secondary antibodies, increasing signal.

Host species primary raised HRP-polymer secondary
Mouse Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)
Rabbit Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)
Rat Goat Anti-Rat IgG H&L (HRP polymer) (ab214882)
Goat Donkey Anti-Goat IgG H&L (HRP polymer) (ab214881)

Biotin HRP-polymer Advantages
Protocol Additional steps for biotin blocking may be required On average 1 hour less Simplified protocol means quicker results with HRP-polymer secondaries.
Sensitivity ♦ ♦ ♦ ♦ ♦ ♦ ♦ Higher number of HRP molecules bound to HRP-polymer antibodies increase their sensitivity
Specificity Potential background from endogenous biotin No background from endogenous biotin Using HRP-polymer secondary antibodies eliminates biotin background.

Great for tissues with high biotin.

Nuclear detection of Ki67 by immunohistochemistry on breast cancer paraffin embedded tissue using Goat Anti-Mouse IgG H&L secondary antibody (HRP polymer) ab214879.

About Abcam

Abcam is a global life sciences company providing highly validated antibodies and other binders and assays to the research and clinical communities to help advance the understanding of biology and causes of disease.

Abcam’s mission is to serve life scientists to help them achieve their mission faster by listening to their needs, continuously innovating and improving and by giving them the tools, data and experience they want. Abcam’s ambition is to become the most influential life science company for researchers worldwide.

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Last updated: May 3, 2019 at 4:26 AM


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