The use of HRP-polymer secondary antibodies is recommended to save time when completing an IHC experiment, to increase sensitivity and to avoid background due to endogenous biotin.
Micropolymer technology is used by HRP-polymer secondary antibodies to form smaller detection complexes that allow better tissue penetration and sensitivity. Also, HRP-polymer secondaries bind more horseradish peroxidase than standard HRP secondary antibodies, increasing signal.
|Host species primary raised
||Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)
||Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)
||Goat Anti-Rat IgG H&L (HRP polymer) (ab214882)
||Donkey Anti-Goat IgG H&L (HRP polymer) (ab214881)
||Additional steps for biotin blocking may be required
||On average 1 hour less
||Simplified protocol means quicker results with HRP-polymer secondaries.
||♦ ♦ ♦
||♦ ♦ ♦ ♦
||Higher number of HRP molecules bound to HRP-polymer antibodies increase their sensitivity
||Potential background from endogenous biotin
||No background from endogenous biotin
||Using HRP-polymer secondary antibodies eliminates biotin background.
Great for tissues with high biotin.
Nuclear detection of Ki67 by immunohistochemistry on breast cancer paraffin embedded tissue using Goat Anti-Mouse IgG H&L secondary antibody (HRP polymer) ab214879.
Abcam is a global life sciences company providing highly validated antibodies and other binders and assays to the research and clinical communities to help advance the understanding of biology and causes of disease.
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