Rabbits possess a special immune system that facilitates the production of antibodies that target small epitopes found in polymers, lipids and small molecules; or to detect epitope changes occurring as the result of single amino acid substitutions or post-translational modifications (PTMs).
RabMAb® primary antibodies provide both high selectivity and strong binding to modified antigens whilst also showing low affinity, i.e. cross reactivity, towards the non-modified antigen.
The ability of rabbit antibodies to identify small epitopes facilitates the identification of PTMs such as acetylation, methylation, phosphorylation and sumoylation. Additionally, many small compounds and peptides do not elicit a good immune response in mice but do so in rabbits.
Abcam can provide more than 500 different PTM specific antibodies, with even more in development. Abcam can also produce custom RabMAb designed to target difficult antibodies. Abcam’s customer service team has an established reputation for the production of antibodies for the identification of PTMs.
Histone H3 modification specific RabMAb primary antibodies:
A. Acetylation: ab76307
B. Methylation: ab32356
C. Phosphorylation: ab32388
RabMAb primary antibodies detecting specific phospho sites:
A. Phosphotyrosine (pY): ab68470
B. Phosphoserine (pS): ab81292
C. Phosphothreonine (pT): ab81551
D. Dual Phospho (pT/pS): ab32525
Custom RabMAb Primary Antibodies Detecting Singly and Doubly Phosphorylated Forms of Protein Kinase A
The data shown below, collected by Dr. Ogut, Division of Cardiovascular Disease, Mayo Clinic, shows RI alpha phospho-specific antibody validation. Abcam used RabMAb technology to produce these two RabMAb primary antibodies which are specific for Ser83 and Ser77 phosphorylated RI alpha.
Reprinted from Archives of Biochemistry and Biophysics, 538 /1, Han YS, Arroyo J, Ogut O., Human heart failure is accompanied by altered protein kinase A subunit expression and post-translational state., 25-33., Copyright (2013), with permission from Elsevier.
Abcam is a global life sciences company providing highly validated antibodies and other binders and assays to the research and clinical communities to help advance the understanding of biology and causes of disease.
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