Optimized Kits for DNA Bisulfite Conversion
When DNA is treated with sodium bisulfite, cytosine residues in the DNA are deaminated to uracil leaving 5-mC residues intact. This alteration in the DNA sequence gives more information about the degree of methylation within a region of DNA to a resolution of a single nucleotide when the bisulfite conversion is followed by sequencing analysis.
Shutterstock | Billion Photos
Bisulfite conversion kits from Abcam are designed to provide optimal and accurate conversion of non-methylated cytosine residues to uracil at a rate of over 99%. The samples suitable for use in the different kits cover a wide range. This allows the array of bisulfite conversion kits to be used in a variety of methylation studies.
The table below provides a glance of the available bisulfite conversion kits to facilitate the choice of the kit that best suits the research on hand.
||Bisulfite Conversion Kit - Whole Cell
||Fast Bisulfite Conversion Kit
||DNA Isolation & Bisulfite Conversion Kit
||Bisulfite-Seq High Sensitivity Kit
||Post-Bisulfite DNA Library Preparation Kit
||Direct bisulfite conversion from cells or tissue
||Bisulfite conversion of purified DNA
||DNA isolation and bisulfite conversion
||Simultaneous bisulfite conversion and fragmentation for Illumina® sequencing
||Fragmentation for Illumina® sequencing after bisulfite conversion
||Cultured cells, tissue samples, embryonic cells / oocytes
||Serum, plasma and body fluids
||Purified bisulfite - converted DNA
|Minimum sample amount
||100 cells / 1 µg tissue
||0.2 ng DNA
||50 pg DNA / 20 cells
||0.5 ng DNA
||1 ng bisulfite-converted DNA
Abcam is a global life sciences company providing highly validated antibodies and other binders and assays to the research and clinical communities to help advance the understanding of biology and causes of disease.
Abcam’s mission is to serve life scientists to help them achieve their mission faster by listening to their needs, continuously innovating and improving and by giving them the tools, data and experience they want. Abcam’s ambition is to become the most influential life science company for researchers worldwide.
Sponsored Content Policy: News-Medical.net publishes articles and related content that may be derived from sources where we have existing commercial relationships, provided such content adds value to the core editorial ethos of News-Medical.Net which is to educate and inform site visitors interested in medical research, science, medical devices and treatments.