Multicolor IHC Detection Kits for Optimal Staining of Antigens

Abcam’s multicolor IHC detection kits, designed for finest staining of two or three antigens on human or animal tissue sections, offer customers the following:

  • Reduced background
    • when staining tissue containing endogenous biotin (for example, liver, kidney and spleen) using an HRP and AP polymer-based system
  • Optimized protocol
    • for simplified arrangement and development of multicolor experiments
  • Better signal to noise ratio
    • for flawless visualization of staining and morphology
  • Flexible color combinations
    • based on brown, red, purple, green, and black
  • Use of rodent antibodies on rodent tissue
    • protocols for blocking endogenous mouse IgG or rat IgG
  • Can be integrated with histology special stains where compatible

Why Enzymatic Staining?

Although multicolor IHC using immunofluorescence is a well-proven method for visualizing numerous antigens, it poses more than a few challenges including:

  • Occurrence of autofluorescence because of formaldehyde fixation
  • Limited combinations with special stains to identify microorganisms or tissue structures
  • Quenching of fluorescent signal at excitation
  • Fading of the fluorescence signal during slide storage

About Abcam

Abcam is a global life sciences company providing highly validated antibodies and other binders and assays to the research and clinical communities to help advance the understanding of biology and causes of disease.

Abcam’s mission is to serve life scientists to help them achieve their mission faster by listening to their needs, continuously innovating and improving and by giving them the tools, data and experience they want. Abcam’s ambition is to become the most influential life science company for researchers worldwide.


Sponsored Content Policy: News-Medical.net publishes articles and related content that may be derived from sources where we have existing commercial relationships, provided such content adds value to the core editorial ethos of News-Medical.Net which is to educate and inform site visitors interested in medical research, science, medical devices and treatments.

Last updated: May 24, 2019 at 7:46 AM

Citations

Please use one of the following formats to cite this article in your essay, paper or report:

  • APA

    Abcam. (2019, May 24). Multicolor IHC Detection Kits for Optimal Staining of Antigens. News-Medical. Retrieved on October 22, 2019 from https://www.news-medical.net/whitepaper/20190524/Multicolor-IHC-Detection-Kits-for-Optimal-Staining-of-Antigens.aspx.

  • MLA

    Abcam. "Multicolor IHC Detection Kits for Optimal Staining of Antigens". News-Medical. 22 October 2019. <https://www.news-medical.net/whitepaper/20190524/Multicolor-IHC-Detection-Kits-for-Optimal-Staining-of-Antigens.aspx>.

  • Chicago

    Abcam. "Multicolor IHC Detection Kits for Optimal Staining of Antigens". News-Medical. https://www.news-medical.net/whitepaper/20190524/Multicolor-IHC-Detection-Kits-for-Optimal-Staining-of-Antigens.aspx. (accessed October 22, 2019).

  • Harvard

    Abcam. 2019. Multicolor IHC Detection Kits for Optimal Staining of Antigens. News-Medical, viewed 22 October 2019, https://www.news-medical.net/whitepaper/20190524/Multicolor-IHC-Detection-Kits-for-Optimal-Staining-of-Antigens.aspx.

Other White Papers by this Supplier