Abcam’s multicolor IHC detection kits, designed for finest staining of two or three antigens on human or animal tissue sections, offer customers the following:
- Reduced background
- when staining tissue containing endogenous biotin (for example, liver, kidney and spleen) using an HRP and AP polymer-based system
- Optimized protocol
- for simplified arrangement and development of multicolor experiments
- Better signal to noise ratio
- for flawless visualization of staining and morphology
- Flexible color combinations
- based on brown, red, purple, green, and black
- Use of rodent antibodies on rodent tissue
- protocols for blocking endogenous mouse IgG or rat IgG
- Can be integrated with histology special stains where compatible
Why Enzymatic Staining?
Although multicolor IHC using immunofluorescence is a well-proven method for visualizing numerous antigens, it poses more than a few challenges including:
- Occurrence of autofluorescence because of formaldehyde fixation
- Limited combinations with special stains to identify microorganisms or tissue structures
- Quenching of fluorescent signal at excitation
- Fading of the fluorescence signal during slide storage
Abcam is a global life sciences company providing highly validated antibodies and other binders and assays to the research and clinical communities to help advance the understanding of biology and causes of disease.
Abcam’s mission is to serve life scientists to help them achieve their mission faster by listening to their needs, continuously innovating and improving and by giving them the tools, data and experience they want. Abcam’s ambition is to become the most influential life science company for researchers worldwide.
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