At least 0.2 ng of DNA is required to build a library.
Each sequencing kit comprises of a full set of reagents optimized for constructing a DNA library in order to carry out next generation sequencing with an Illumina® sequencer. These kits may be used for applications such as genomic DNA-Seq, ChIP-Seq, MeDIP/hMeDIP-Seq, bisulfite-Seq or targeted re-sequencing.
The Abcam range of products is given below:
High-Sensitivity DNA Library Preparation kit (ab185905)
This allows the researcher to build libraries using 0.2 ng or more of DNA input.
The time required for the protocol to be performed is 1.5 hours.
DNA Library Preparation kit (ab185903)
This kit can be used with DNA inputs between 5 ng to 1 ug.
The time required for the protocol to be performed is 2.5 hours.
Post-Bisulfite DNA Library Preparation Kit (ab185906)
With this kit, a library can be built with less than one nanogram of input DNA following bisulfite conversion.
The time taken for the protocol is 5 hours.
Abcam is a global life sciences company providing highly validated antibodies and other binders and assays to the research and clinical communities to help advance the understanding of biology and causes of disease.
Abcam’s mission is to serve life scientists to help them achieve their mission faster by listening to their needs, continuously innovating and improving and by giving them the tools, data and experience they want. Abcam’s ambition is to become the most influential life science company for researchers worldwide.
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