When recombinant rabbit monoclonal antibodies can be used in BSA and in azide-free formats, it makes antibody-labeling and functional assays simple to perform.
The standard range of RabMAb® antibodies is stored in a solution of phosphate-buffered saline (PBS), bovine serum albumin (BSA), glycerol, and sodium azide. BSA and sodium azide are required to keep the antibody stable and to prevent contamination of the antibody. They can also prevent proper conjugation of the antibody to fluorochomes, metals and enzymes.
BSA is a competitive inhibitor of some enzymes or dyes, preventing the binding of the primary antibody to them. This means a significant fall in the efficiency of conjugation.
Sodium azide is sometimes toxic to the cells in an antibody solution. This restricts the usefulness of the antibody solution in cell culture. Furthermore, sodium azide inhibits horseradish peroxidase (HRP) activity, and can prevent antibody conjugation.
Abcam has a range of reformulated recombinant monoclonal antibodies, designed to avoid these issues, as well as easy to use kits that can be employed to eliminate BSA and sodium azide from the solution.
Advantages of PBS-only RabMAb antibodies
RabMAb antibodies with PBS-only format are:
- Free of BSA, azide and glycerol
- Selected products contain very low endotoxin levels, confirmed by testing
- Come in a 100 µg (1 mg/mL) size
- Are always ready to ship and available in stock
ab209897: Recombinant rabbit monoclonal anti-Ki67 antibody (green), staining Ki67 in wild-type HAP1 cells (top panel) and Ki67-knockout HAP1 cells (bottom panel).
ab199376: Rabbit monoclonal IgG - Isotype control (black) utilized as a negative control in flow cytometry analysis of fixed Jurkat cells. ab199376 shows no signal overlap.
Abcam is a global life sciences company providing highly validated antibodies and other binders and assays to the research and clinical communities to help advance the understanding of biology and causes of disease.
Abcam’s mission is to serve life scientists to help them achieve their mission faster by listening to their needs, continuously innovating and improving and by giving them the tools, data and experience they want. Abcam’s ambition is to become the most influential life science company for researchers worldwide.
Sponsored Content Policy: News-Medical.net publishes articles and related content that may be derived from sources where we have existing commercial relationships, provided such content adds value to the core editorial ethos of News-Medical.Net which is to educate and inform site visitors interested in medical research, science, medical devices and treatments.