Tools for Metabolic Research Involving ADP, NADH and More

ATP, as a form of energetic cellular currency, is arguably the most important small molecule in the metabolism. As a result, assays of ATP and other similar molecules such as ADP, NADH and its reduced and phosphorylated forms, are important for cellular and metabolic research.

Assays of ATP can either be based on using the ATP-dependent phosphorylation of glyercol (or similar) to produce a product, which is then measured, or by extracting cellular ATP following its production using light and ATP-dependent luciferase.

Assay Read out Notes Assay kits
ATP Plate reader No-wash assay. ab113849
ADP/ATP Plate reader No-wash assay. Same method as ATP assay. After ATP analysis, ADP is converted to ATP and detected. ab65313
ATP Plate reader Used with cell lysates.
Not as sensitive as luminescence assays.
Phosphate Plate reader ab65622, ab219938, ab102508
Pyrophosphate Plate reader ab112155, ab179836

​​​I personally recommend ab113849 to measure extracellular ATP levels for its ease of use and for the kindly attention and help I received from the Abcam scientific support specialists.

Dr. Lisa Sevenich


Assay Readout Assay kits
NADH Plate reader ab186030
NAD/NADH Plate reader ab65348, ab176723, ab186032
NADP / NADPH Plate reader ab186033, ab65349, ab176724
NADPH Plate reader ab186031

About Abcam

Abcam is a global life sciences company providing highly validated antibodies and other binders and assays to the research and clinical communities to help advance the understanding of biology and causes of disease.

Abcam’s mission is to serve life scientists to help them achieve their mission faster by listening to their needs, continuously innovating and improving and by giving them the tools, data and experience they want. Abcam’s ambition is to become the most influential life science company for researchers worldwide.

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Last updated: Jun 6, 2019 at 11:47 AM


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