ATP, as a form of energetic cellular currency, is arguably the most important small molecule in the metabolism. As a result, assays of ATP and other similar molecules such as ADP, NADH and its reduced and phosphorylated forms, are important for cellular and metabolic research.
Assays of ATP can either be based on using the ATP-dependent phosphorylation of glyercol (or similar) to produce a product, which is then measured, or by extracting cellular ATP following its production using light and ATP-dependent luciferase.
||No-wash assay. Same method as ATP assay. After ATP analysis, ADP is converted to ATP and detected.
||Used with cell lysates.
Not as sensitive as luminescence assays.
||ab65622, ab219938, ab102508
I personally recommend ab113849 to measure extracellular ATP levels for its ease of use and for the kindly attention and help I received from the Abcam scientific support specialists.
Dr. Lisa Sevenich
NAD Assays - NADH, NAD, NADHP and NADP
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