Enhancing Marker Expression with Sensory Maturation Maximizer Supplement

Maturation Maximizer is a completely defined media supplement developed to improve the current sensory neuron maintenance medium (ax0060). It functions by imitating in-vitro cell-to-cell communication and interaction factors, which take place when these cells are in their intrinsic environment.

The supplement is recognized to hold factors existing in the peripheral nervous system, and specifically, the peripheral environment of sensory neurons.

So, how does this help in enhancing functionality and time to assay?

Significantly Enhanced Marker Expression

The media supplement enables an average increase of 30% –45% for neurons expressing TRPv1, Nav1.7, and Nav1.8.

Faster Maturation, Quicker Results

Time to maturation of sensory neurons is reduced by more than 40 days.

  • Infection potential is considerably reduced through extended culture
  • 10 hours less labor
  • 2 less full-media changes
  • 15 less half-media changes

Sensory Biomarker Expression 30 Days Post Plating

In addition to carrying the same activation, the new supplemented sensory neurons also carry the expression state of sensory neurons in-vivo to a certain extent—for example, the expression markers TRPv1, TRPa1, TRPv2, and TRPm8.

Functional Characterization

Kinetic states of TRPv1 are in a rapid-response inward rectifying state to antagonists like Capsaicin. Exploiting this new system also promotes faster development and earlier initiation of maturity.

    Cumulative percentage of ROIs responding to capsaicin
Condition # of ROIs 1 μM 10 μM 100 μM Non-responder
Control 71 19 43 43 57
Supplement 78 91 94 99 1

 

DeltaF/Fo values for the control and supplemented neurons treated with the vehicle control and 1 µM capsaicin after 20 days in-vitro.

DeltaF/Fo values for the control and supplemented neurons treated with the vehicle control and 1 µM capsaicin after 20 days in-vitro.

The MATURATION MAXIMIZER supplement promotes an improved physiological model in response to capsaicin, taking place at 1 µM for 90% of the sensory neuron population.

Available Products

Sensory MATURATION MAXIMIZER Medium Kit (ax0158)

Sensory Neuron Maintenance Medium (ax0060) + Maturation Maximizer Supplement.

Sensory Neuron Progenitor MAXIMIZER Kit (ax00157)

Sensory Neuron Progenitor Kit (ax0057) + Maturation Maximizer Supplement.

 

About AXOL Biosciences

Axol specializes in human cell culture.

Axol produces high quality human cell products and critical reagents such as media and growth supplements. We have a passion for great science, delivering epic support and innovating future products to help our customers advance faster in their research.

Our expertise includes reprogramming cells to iPSCs and then differentiating to various cell types. We supply differentiated cells derived from healthy donors and patients of specific disease backgrounds. As a service, we also take cells provided by customers (primary or iPSC) and then do the reprogramming (when necessary) and differentiation. Clearly, by offloading the burden of generating cells, your time is freed up to focus on the research. Axol holds the necessary licenses that are required to do iPSC work.

The package wouldn't be complete without optimized media, coating solutions and other reagents. Our in-house R&D team works hard to improve on existing media and reagents as well as innovate new products for human cell culture. We also supply a growing range of human primary cells; making Axol your first port of call for your human cell culture needs.


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Last updated: Jul 15, 2019 at 10:18 AM

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