Quantification and Detection of Phospholipids

Phospholipids are key components of the bilayers of all plasma membranes. For example, Phosphatidylethanolamine is responsible for membrane stabilization, membrane fission/fusion, and is involved in the secretion of very-low-density lipoproteins, while Cardiolipin makes up around 20% of mitochondria total lipid composition.

BioVision offer more than eight specific, yet versatile, products for the quantification and detection of phospholipids in tissue lysates, or intact mammalian cells (EZClick™ Global Phospholipid Synthesis Assay Kit), Cardiolipin, Phosphatidylcholine, Phosphatidylglycerol, Phosphatidylethanolamine, Phosphatidylserine, Phosphatidylinositol, and Phospholipid Assay Kits are accurate, simple, and reproducible products which can measure diverse phospholipids in biological samples like exosomes, serum, and plasma. These kits permit analyses of biosynthesis, subcellular localization, and quantification of these molecules in tissues/cells/biofluids.  

Key Features

  • Convenient: minimal sample preparation, fast protocols (less than 2 hours)
  • Sensitive: fluorometric format
  • Adaptable: works with most of the commonly available instrumentations
  • Non-radioactive, versatile assays (Flow cytometry/Fluorescence Microscopy/Microplate)
  • Specific, homogeneous assay
  • Cost-effective: 100 assays; High Throughput Screening (HTS) compatible

Figure:  (A) Specificity for detection of phosphatidylserine (PS) over other common phospholipids (K565-100). At a 5-fold molar excess (5 nmoles) vs PS (1 nmole), phosphatidic acid (PA) contributes ≤10% interference while phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) contribute ≤5%. (B). Estimation of Phosphatidic Acid using  (K748-100) in lipid extracts of lysates from rat neocortex (2 µl extract, 200 µg/µl wet brain tissue) and 3T3-L1 cells (2 µl extract, 100000 cells/µl). Lipid extraction was carried out as described in K748-100 protocols.

About BioVision Incorporated

BioVision, Inc., is a privately held Life Science company headquartered in the beautiful San Francisco Bay Area.

BioVision develops and offers a wide variety of products including assay kits, antibodies, recombinant proteins & enzymes, and other innovative research tools for studying Apoptosis, Metabolism, Cell Proliferation, Cellular Stress, Cell Damage and Repair, Diabetes, Obesity and Metabolic Syndrome, Stem Cell Biology, Gene Regulation, Signal Transduction, etc. BioVision's products are currently being sold in more than 60 countries worldwide.

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Last updated: Feb 21, 2020 at 9:30 AM


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