The goal is to establish whether a wound dressing infused with silver, iodine, or Manuka honey will reduce the growth of C. albicans and S. aureus bacteria when creating a polymicrobial biofilm on a wounded Labskin.
- Using a biopsy punch, all Labskin samples were wounded and infected immediately with a combination of C. albicans and S. aureus.
- To promote the formation of a biofilm, all samples were incubated for a period of 48 hours.
- Post 48 hours, wound dressings were applied directly over the wound site in a few samples, which were subsequently incubated for another 72 hours.
- Microbial viable counting was used to assess the samples.
Figure 1. Biofilm formation on wounded Lab-skin and PAS staining. (A) Polymicrobial biofilm growing in wounded Labskin4.5 (B). PAS staining showing a biofilm infecting a wound (10x). (C) High magnification of biofilm showing yeast and bacteria growing together (100x).
Figure 2. Application of wound dressing after 48 hours of biofilm formation.
Figure 3. Viable counts after 72 hours of treatment Log10 difference compared to untreated control.
Polymicrobial and monomicrobial biofilms were effectively developed in the Labskin wounded model. Fungi, bacteria, or a combination of both microorganisms were used to infect the wounds.
Only the iodine-impregnated wound dressing was observed to have an evident antimicrobial effect.
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