Assessing Wound Dressings on Biofilm Infections

The goal is to establish whether a wound dressing infused with silver, iodine, or Manuka honey will reduce the growth of C. albicans and S. aureus bacteria when creating a polymicrobial biofilm on a wounded Labskin.

Method

  • Using a biopsy punch, all Labskin samples were wounded and infected immediately with a combination of C. albicans and S. aureus.
  • To promote the formation of a biofilm, all samples were incubated for a period of 48 hours.
  • Post 48 hours, wound dressings were applied directly over the wound site in a few samples, which were subsequently incubated for another 72 hours.
  • Microbial viable counting was used to assess the samples.

Results

Biofilm formation on wounded Lab-skin and PAS staining. (A) Polymicrobial biofilm growing in wounded Labskin4.5 (B). PAS staining showing a biofilm infecting a wound (10x). (C) High magnification of biofilm showing yeast and bacteria growing together (100x).

Figure 1. Biofilm formation on wounded Lab-skin and PAS staining. (A) Polymicrobial biofilm growing in wounded Labskin4.5 (B). PAS staining showing a biofilm infecting a wound (10x). (C) High magnification of biofilm showing yeast and bacteria growing together (100x).

Application of wound dressing after 48 hours of biofilm formation.

Figure 2. Application of wound dressing after 48 hours of biofilm formation.

Viable counts after 72 hours of treatment Log10 difference compared to untreated control.

Figure 3. Viable counts after 72 hours of treatment Log10 difference compared to untreated control.

Summary

Polymicrobial and monomicrobial biofilms were effectively developed in the Labskin wounded model. Fungi, bacteria, or a combination of both microorganisms were used to infect the wounds.

Only the iodine-impregnated wound dressing was observed to have an evident antimicrobial effect.

About Labskin

At Labskin we deliver human skin microbiology services to support your product R&D activities in the cosmetic, personal care, medical device and pharmaceutical sectors. With our sector experience and use of technology, you will be accessing industry-focused services supported by world-leading skin science expertise.

Whether you need rapid, focused analysis or flexible, tailor-made research programs we can help you develop and validate skincare ingredients and products which really work.

Our skin model is a 3D human skin equivalent that incorporates vital biological components to model normal skin function.

Developed over 12 years with more than 30 scientific journal publications, it is made from young keratinocytes (human skin cells) and adult fibroblasts (metabolically-active, collagen-producing human skin cells).

“An ideal platform for basic or applied skin research, testing compounds or formulated products for the cosmetic, pharmaceutical and chemical sectors.”


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Last updated: Oct 28, 2019 at 4:43 AM

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