The Release of Pro-Inflammatory Cytokines by Labskin

The aim of this article is to quantify the Interleukins IL-1α and IL-23 released by Labskin.

The uncolonized Labskin is compared with the Labskin colonized with S. aureus bacteria (post-inoculation of 6 hours and 24 hours), P. acnes, C. albicans, S. epidermidis, 3x mix Normal microflora, as well as wounds infected with Interkingdom biofilms.

Method

Unwounded Labskin was initially colonized with C. albicans, P. acnes, S. epidermidis, S. aureus, or a combination of normal skin microflora. The colonized Labskin was then incubated for a period of 24 hours. After 24 hours, Labskin medium was taken and the amount of cytokines was evaluated by ELISA.

The wounded Labskin was then infected with three mixes comprising varied numbers of C. albicans and S. aureus. For cytokine assessment, Labskin medium was taken after a period of 24 and 48 hours.

Results

  • After 24 hours, trace amounts of IL-1α were found but only in the Labskin colonized by either P. acnes or C. albicans. Again after 24 hours, a small amount of IL-23 was found when Labskin was colonized with individual microorganisms. But when Labskin was colonized by the combination of three microorganisms, that amount was raised from below 10 pg/mL up to 40 pg/mL.
  • High levels of IL-1α produced by all wounded Labskin increased with the number of microorganisms added. Uninfected wounds contained an insignificant amount of IL-23. High levels of IL-23 produced by all infected wounds increased with the number of microorganisms added.

Comparison between IL-1α and IL-23 production by Labskin1.1 colonized with several microorganisms.

Figure 1. Comparison between IL-1α and IL-23 production by Labskin1.1 colonized with several microorganisms.

Comparison between IL-1α and IL-23 production by wounded Labskin4.5 infected with a mix of S. aureus and C. albicans.

Figure 2. Comparison between IL-1α and IL-23 production by wounded Labskin4.5 infected with a mix of S. aureus and C. albicans.

Summary

To sum up, the species and the number of microbes in contact with the Labskin regulate the production of IL-23, while a different type of insults is likely to regulate the production of IL-1α.

About Labskin

At Labskin we deliver human skin microbiology services to support your product R&D activities in the cosmetic, personal care, medical device and pharmaceutical sectors. With our sector experience and use of technology, you will be accessing industry-focused services supported by world-leading skin science expertise.

Whether you need rapid, focused analysis or flexible, tailor-made research programs we can help you develop and validate skincare ingredients and products which really work.

Our skin model is a 3D human skin equivalent that incorporates vital biological components to model normal skin function.

Developed over 12 years with more than 30 scientific journal publications, it is made from young keratinocytes (human skin cells) and adult fibroblasts (metabolically-active, collagen-producing human skin cells).

“An ideal platform for basic or applied skin research, testing compounds or formulated products for the cosmetic, pharmaceutical and chemical sectors.”


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Last updated: Oct 28, 2019 at 4:42 AM

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