Using Different Wound Dressings on a Living Skin Equivalent

This article aims to establish whether a wound dressing impregnated with silver or iodine will prevent S. aureus bacteria from penetrating the dermal layer in wounded and infected Labskin.

Another objective is to evaluate the wound healing characteristics of the wound dressing impregnated with silver and iodine.

Method

  • Initially, a scalpel blade was used to wound each Labskin sample, and then a few samples were instantly infected with S. aureus.
  • Each sample was incubated for a period of 24 hours.
  • Post 24 hours, wound dressings were directly applied over the wound site in a few samples. These samples were subsequently incubated for another 24 hours to make them ready for analysis.
  • Through histology, the samples were assessed, sectioned to a size of 5 μm, and stained with hematoxylin and eosin (H&E).

Results

  • After 24 hours of incubation, the wound dressing impregnated with silver and iodine was able to reduce the penetration of S. aureus into the dermis, when compared to samples that lacked the wound dressing.
  • The iodine wound dressing inhibited the migration of keratinocytes into the wound site, while the wound dressing impregnated with silver did not have any impact on the migration of keratinocytes.

H&E images of Labskin inoculated with S. aureus for 24 hours and then a wound dressing applied for 24 hours. (A) H&E images of Labskin inoculated with S. aureus and treated with wound dressing, (B) H&E images of Labskin inoculated with S. aureus, and (C) H&E images of Labskin cultured with just the wound dressing.

Figure 1. H&E images of Labskin inoculated with S. aureus for 24 hours and then a wound dressing applied for 24 hours. (A) H&E images of Labskin inoculated with S. aureus and treated with wound dressing, (B) H&E images of Labskin inoculated with S. aureus, and (C) H&E images of Labskin cultured with just the wound dressing.

Summary

Both types of wound dressings were able to reduce the infection of S. aureus into the dermal layer of Labskin and thus reduced the bacteria’s cytotoxic effect.

However, compared to the silver-impregnated wound dressing, the iodine wound dressing prevented the migration of keratinocytes. Hence, the silver-impregnated wound dressing could be more beneficial in supporting the wound healing process in an infected wound setting.

About Labskin

At Labskin we deliver human skin microbiology services to support your product R&D activities in the cosmetic, personal care, medical device and pharmaceutical sectors. With our sector experience and use of technology, you will be accessing industry-focused services supported by world-leading skin science expertise.

Whether you need rapid, focused analysis or flexible, tailor-made research programs we can help you develop and validate skincare ingredients and products which really work.

Our skin model is a 3D human skin equivalent that incorporates vital biological components to model normal skin function.

Developed over 12 years with more than 30 scientific journal publications, it is made from young keratinocytes (human skin cells) and adult fibroblasts (metabolically-active, collagen-producing human skin cells).

“An ideal platform for basic or applied skin research, testing compounds or formulated products for the cosmetic, pharmaceutical and chemical sectors.”


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Last updated: Oct 28, 2019 at 4:42 AM

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