The aim of this article is to establish the rate at which the stratum corneum (SC) forms in Labskin1.1, with up to 30 days in air-liquid interface (ALI).
Labskin was initially fixed in duplicate on days 5, 7, 9, 12, 14, 16, 19, 21, 23, 26, 28, and 30 in ALI. The sections were then stained with H&E and the thickness of the stratum corneum was measured in the middle. Each end of the section was also measured with the help of the Image J software.
Figure 1. H&E images of the Labskin sections show that the stratum corneum gets thicker with time.
As the stratum corneum becomes thicker, the epidermis of the Labskin reduces over time.
Figure 2. The stratum corneum gets thicker as the Labskin ages. Variability in measurement is due to the separation of the SC.
From day 5 to day 12 in ALI, the Labskin exhibited a thick epidermis. As the days continue, the SC becomes thicker and the epidermis gets thinner.
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