Using Labskin to Show Formation Rate of the Stratum Corneum

The aim of this article is to establish the rate at which the stratum corneum (SC) forms in Labskin1.1, with up to 30 days in air-liquid interface (ALI).

Method

Labskin was initially fixed in duplicate on days 5, 7, 9, 12, 14, 16, 19, 21, 23, 26, 28, and 30 in ALI. The sections were then stained with H&E and the thickness of the stratum corneum was measured in the middle. Each end of the section was also measured with the help of the Image J software.

Results

H&E images of the Labskin sections show that the stratum corneum gets thicker with time.

Figure 1. H&E images of the Labskin sections show that the stratum corneum gets thicker with time.

As the stratum corneum becomes thicker, the epidermis of the Labskin reduces over time.

The stratum corneum gets thicker as the Labskin ages. Variability in measurement is due to the separation of the SC.

Figure 2. The stratum corneum gets thicker as the Labskin ages. Variability in measurement is due to the separation of the SC.

Summary

From day 5 to day 12 in ALI, the Labskin exhibited a thick epidermis. As the days continue, the SC becomes thicker and the epidermis gets thinner.

About Labskin

At Labskin we deliver human skin microbiology services to support your product R&D activities in the cosmetic, personal care, medical device and pharmaceutical sectors. With our sector experience and use of technology, you will be accessing industry-focused services supported by world-leading skin science expertise.

Whether you need rapid, focused analysis or flexible, tailor-made research programs we can help you develop and validate skincare ingredients and products which really work.

Our skin model is a 3D human skin equivalent that incorporates vital biological components to model normal skin function.

Developed over 12 years with more than 30 scientific journal publications, it is made from young keratinocytes (human skin cells) and adult fibroblasts (metabolically-active, collagen-producing human skin cells).

“An ideal platform for basic or applied skin research, testing compounds or formulated products for the cosmetic, pharmaceutical and chemical sectors.”


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Last updated: Oct 28, 2019 at 4:42 AM

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