To determine the immediate and residual effects that the application of ingredients to treat dandruff to Labskin full thickness living skin equivalent has on the viability of Malassezia globosa and Malassezia restricta.
- Products were applied to the Labskin surface pre-colonized to examine immediate anti-fungal activity for 24 hours with Malassezia globosa and Malassezia restricta in GS-24. The surface of the skin was washed after 2 minutes exposure using a modified scrub wash with neutralizer and viable Malassezia enumerated on RM-SMA agar medium.
- Products were applied to the Labskin surface for 2 minutes and then washed to remove them in order to examine residual anti-fungal activity. The surface of the skin was then colonized with Malassezia globosa and Malassezia restricta in GS-24 and incubated. The skin surface was washed after 3 hours using a modified scrub wash and viable Malassezia enumerated.
Figure 1. Budding Malassezia on LabSkin
A modified scrub wash technique was used to recover Malassezia from the surface of LabSkin and a specially formulated RM--SMA growth medium was used to enumerate viable cells enumerated.
Materials are presented in solution in classic in vitro antimicrobial testing protocols (MIC, MBC etc.).The growth of Malassezia in a phenotypically- relevant manner is supported with Labskin as it provides a living, skin-equivalent testing surface.
Ingredients and formulations can be evaluated with the Labskin model and benchmarked against products of recognized clinical activity. Enhanced predictivity can help to de-risk the move from in vitro screening to clinical assessment.
At Labskin we deliver human skin microbiology services to support your product R&D activities in the cosmetic, personal care, medical device and pharmaceutical sectors. With our sector experience and use of technology, you will be accessing industry-focused services supported by world-leading skin science expertise.
Whether you need rapid, focused analysis or flexible, tailor-made research programs we can help you develop and validate skincare ingredients and products which really work.
Our skin model is a 3D human skin equivalent that incorporates vital biological components to model normal skin function.
Developed over 12 years with more than 30 scientific journal publications, it is made from young keratinocytes (human skin cells) and adult fibroblasts (metabolically-active, collagen-producing human skin cells).
“An ideal platform for basic or applied skin research, testing compounds or formulated products for the cosmetic, pharmaceutical and chemical sectors.”
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