Examining Performance of Anti-Ageing Cosmetic Ingredients


To determine the effect that repeated application to Labskin full thickness living skin of Retin-A along with a consumer emollient equivalent has on procollagen I production in dermal fibroblasts by immunohistochemistry (IHC).


  • Over a two-week period products were applied to the surface of Labskin each day
  • Each time 20 μL of product was pipetted onto the surface and a sterile glass rod was used to spread it
  • From each Labskin unit 3 x 8 mm punch biopsies were removed after treatment
  • Samples were formalin fixed and paraffin embedded for histology
  • To examine the expression of Procollagen-1 in dermal fibroblasts sections of 5 μm were prepared and immuno- histochemistry was carried out


Image scoring ws used to assess Procollagen I immunolabelling associated with fibroblasts within the dermis of Labskin.

Affect of products on procollagen production

Figure 1. Affect of products on procollagen production


Anti-aging claims for cosmetics ingredients and formulations can be supported by assessing procollagen production by immuno-histochemistry (IHC) in dermal fibroblasts in Labskin full thickness living skin equivalent when benchmarked against products of recognized activity i.e. Retin-A.

Dermal Fibroblast Labelling Scoring

Labskin can be used within the same experimental design to simultaneously evaluate multiple factors including cytokine responses (i.e. IL-1a, PGE2, TNFa, IL-10 etc.), histological changes, wound repair and photoreactivity in addition to skin commensal and pathogenic microorganisms.

About Labskin

At Labskin we deliver human skin microbiology services to support your product R&D activities in the cosmetic, personal care, medical device and pharmaceutical sectors. With our sector experience and use of technology, you will be accessing industry-focused services supported by world-leading skin science expertise.

Whether you need rapid, focused analysis or flexible, tailor-made research programs we can help you develop and validate skincare ingredients and products which really work.

Our skin model is a 3D human skin equivalent that incorporates vital biological components to model normal skin function.

Developed over 12 years with more than 30 scientific journal publications, it is made from young keratinocytes (human skin cells) and adult fibroblasts (metabolically-active, collagen-producing human skin cells).

“An ideal platform for basic or applied skin research, testing compounds or formulated products for the cosmetic, pharmaceutical and chemical sectors.”

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Last updated: Oct 28, 2019 at 4:42 AM


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