Investigating Dermatophyte Colonization with Lab Grown Skin

Objective

To evaluate anti-dermatophyte treatments through the development of methods for the reproducible invasion of Labskin with Trichophyton rubrum.

Method

  • A 5 day incubation period was in place for Labskin in the presence of the fungus, at 37 oC in 5% CO2
  • 2.5 x 104 cm-2 conidia of Trichophyton rubrum was applied to 3 Labskin pre-treated inserts
  • At day 4 3 Labskin inserts were inoculated and treated with product once
  • Once at day 2 and once at day 4, 3 Labskin inserts were inoculated and treated with product twice
  • At day 5, in order to conduct colony counting 5 mm biopsy punches were removed from the Labskin
  • Samples were formalin fixed and paraffin embedded for histology
  • To visualize fungal cells haematoxylin and Periodic Acid Schiff (PAS) stains were used

Results

In all inoculated samples fungal colonisation of the epidermis was observed. When treatment schedules were compared differences in product efficacy were detected.

Activity of topical antifungal treatments A & B against Trichophyton rubrum on Labskin

Figure 1. Activity of topical antifungal treatments A & B against Trichophyton rubrum on Labskin

Summary

Dermatophyte Trichophyton rubrum effectively colonized the Labskin. Fungal hyphae interspersed layers of cornified tissue four days post-inoculation. However, the tissue underneath stayed intact, which makes the model perfect for studying anti- dermatophyte treatments.

Trichophyton rubrum colonizing Labskin 4 days after inoculation. Stained with Periodic Acid Schiff & Heamotoxylin.

Figure 2. Trichophyton rubrum colonizing Labskin 4 days after inoculation. Stained with Periodic Acid Schiff & Heamotoxylin.

About Labskin

At Labskin we deliver human skin microbiology services to support your product R&D activities in the cosmetic, personal care, medical device and pharmaceutical sectors. With our sector experience and use of technology, you will be accessing industry-focused services supported by world-leading skin science expertise.

Whether you need rapid, focused analysis or flexible, tailor-made research programs we can help you develop and validate skincare ingredients and products which really work.

Our skin model is a 3D human skin equivalent that incorporates vital biological components to model normal skin function.

Developed over 12 years with more than 30 scientific journal publications, it is made from young keratinocytes (human skin cells) and adult fibroblasts (metabolically-active, collagen-producing human skin cells).

“An ideal platform for basic or applied skin research, testing compounds or formulated products for the cosmetic, pharmaceutical and chemical sectors.”


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Last updated: Oct 28, 2019 at 4:42 AM

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