To evaluate the effect on the growth of normal vaginal commensal Lactobacillus acidophilus and the growth and TSST-1 production by pathogenic Staphylococcus aureus through an in vitro safety assessment of tampon material.
- Liquid cultures of Lactobacillus acidophilus and Staphylococcus aureus were used to incubate materials for 24 hours.
- Culture optical density and viable cell counts were used to assess growth at regular intervals. The effect of growth on culture pH was also monitored.
- Toxic Shock Syndrome Toxin 1 (TSST-1 ) was also assessed by taking and processing samples at regular intervals.
- A validated in-house ELISA was used to quantify TSST-1 was quantified.
Figure 1. Growth of Staphylococcus aureus in the presence of test tampon material
Figure 2. TSST-1 in culture supernatants of Staphylococcus aureus in the presence of test tampon material
Figure 3. Growth of Lactobacillus acidophilus in the presence of test tampon material
Figure 4. Change in growth medium pH for S. aureus (SA) and L. acidophilus (LA) in the presence of test tampon material
Safety claims for hygienic tampons are supported through this assessment of the growth and bioactivity of vaginal commensal and pathogenic microorganisms when benchmarked against existing products.
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