Using Matrix Assisted Laser Desorption Ionisation Mass Spectrometry Imaging (MALDI-MSI), the objective was to examine whether adding a penetration enhancer to a terbinafine product formulation can increase terbinafine penetration into Labskin.
- Terbinafine hydrochloride (5 mg/mL) in actone/olive oil (80:20 v/v) was used to treat Labskin over 24 hours with or without a penetration enhancer.
- In preparation for mass spectrometry imaging, samples were flash frozen.
- Frozen samples were sectioned (10 μm), coated with MALDI matrix (α-cyano-4-hydroxycinnamic acid) and an analysis for m/z ratios associated with terbinafine (m/z 292.2 and m/z 141) was carried out.
When the penetration enhancer was added to the production formulation there was increased Terbinafine in the Labskin.
Figure 1. Sections of Labskin treated with Terbinafine without (Tissue 1) or with (Tissue 2) a penetration enhancer
Figure 2. Graph showing intensity data for terbinafine in Labskin without (3.41± 0.61 mg/g of tissue) or with (4.2 ± 0.813 mg/g of tissue) the penetration enhancer
The penetration of ingredients and formulations can be assessed and quantified using the combination of MALDI-MSI with Labskin and benchmarked against products of recognized activity over time.
Schematic Representation of the Mass Spectrometry Imaging Workflow
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