Investigating Drug Penetration in Labskin

Objective

Using Matrix Assisted Laser Desorption Ionisation Mass Spectrometry Imaging (MALDI-MSI), the objective was to examine whether adding a penetration enhancer to a terbinafine product formulation can increase terbinafine penetration into Labskin.

Methods

  • Terbinafine hydrochloride (5 mg/mL) in actone/olive oil (80:20 v/v) was used to treat Labskin over 24 hours with or without a penetration enhancer.
  • In preparation for mass spectrometry imaging, samples were flash frozen.
  • Frozen samples were sectioned (10 μm), coated with MALDI matrix (α-cyano-4-hydroxycinnamic acid) and an analysis for m/z ratios associated with terbinafine (m/z 292.2 and m/z 141) was carried out.

Results

When the penetration enhancer was added to the production formulation there was increased Terbinafine in the Labskin.

Sections of Labskin treated with Terbinafine without (Tissue 1) or with (Tissue 2) a penetration enhancer

Figure 1. Sections of Labskin treated with Terbinafine without (Tissue 1) or with (Tissue 2) a penetration enhancer

Graph showing intensity data for terbinafine in Labskin without (3.41± 0.61 mg/g of tissue) or with (4.2 ± 0.813 mg/g of tissue) the penetration enhancer

Figure 2. Graph showing intensity data for terbinafine in Labskin without (3.41± 0.61 mg/g of tissue) or with (4.2 ± 0.813 mg/g of tissue) the penetration enhancer

Summary

The penetration of ingredients and formulations can be assessed and quantified using the combination of MALDI-MSI with Labskin and benchmarked against products of recognized activity over time.

Schematic Representation of the Mass Spectrometry Imaging Workflow

About Labskin

At Labskin we deliver human skin microbiology services to support your product R&D activities in the cosmetic, personal care, medical device and pharmaceutical sectors. With our sector experience and use of technology, you will be accessing industry-focused services supported by world-leading skin science expertise.

Whether you need rapid, focused analysis or flexible, tailor-made research programs we can help you develop and validate skincare ingredients and products which really work.

Our skin model is a 3D human skin equivalent that incorporates vital biological components to model normal skin function.

Developed over 12 years with more than 30 scientific journal publications, it is made from young keratinocytes (human skin cells) and adult fibroblasts (metabolically-active, collagen-producing human skin cells).

“An ideal platform for basic or applied skin research, testing compounds or formulated products for the cosmetic, pharmaceutical and chemical sectors.”


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Last updated: Oct 28, 2019 at 4:42 AM

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