Scaling anchorage-dependent cells for allogenic cell therapies

To establish cell lines, all cell culturing procedures require a primary cell culture. Cells exist in one of two forms when it comes to preparing these cultures: anchorage-dependent or suspension cells. 

Image Credit: ShutterStock/Anusorn Nakdee

Anchorage-dependent primary cells need to adhere to a solid support to thrive and form adherent cultures, whereas suspension primary cells do not (cells are suspended in the culture media).

It is often assumed that suspension culture is the only viable alternative for achieving significant scale; however, in many instances adherent scale-up is a more effective option (for example, when the primary cells are of a biological origin that favors adherence).

There are numerous adherent platforms available, ranging from standard cell culture flasks and multi-layered vessels to fixed-bed bioreactors. And there are microcarriers, a kind of hybrid of adherent and suspension in which adherent cells attached to beads suspended in culture media.

Why are primary cell cultures necessary for establishing cell lines?

Figure 1. Image Credit: Corning Life Sciences

Waisman Biomanufacturing - whose cell therapy services include work with mesenchymal stromal cells (MSCs), human embryonic stem cell (hESC) banks, and a variety of other allogeneic cell therapy lines (e.g., lung cancer cell line) for human cell therapy applications - recently ramped up capacity at its Madison, Wisconsin manufacturing plant.

The company concentrated its expansion on building larger scaled manufacturing processes for allogeneic cell therapeutics, and, like any other cell therapy solution provider, they focused on ensuring scalability and high yield at an acceptable cost and within the shortest possible timeline. Waisman chose Corning® HYPERStack®-36 vessels, an adherent, closed system cell culture platform that optimizes the ratio of growth surface area to volumetric footprint and allows for a more rapid and effective scale-up process.

About Corning Life Sciences

A division of Corning Incorporated, Corning Life Sciences is a leading global manufacturer of cell culture products and solutions that enable academic, biotech and biopharma scientists to harness the power of cells to create life-changing innovations. Corning supports a range of application areas including core cell culture, 3D cell culture, bioprocess, cancer research, primary and stem cell research, drug screening, cell and gene therapy, disease modeling, lab automation and more.

Whether your goal is stem cell expansion or viral vector production, Corning Life Sciences platforms, including HYPERStack vessels that maximize cell growth area in a small footprint, the high-yield Ascent® Fixed Bed Reactor platform, microcarriers, and closed system solutions can help get you there. Choose from hundreds of vessels, the widest selection of cell culture surfaces, and custom media in a variety of single-use technology configurations. Learn more at

Last updated: Nov 16, 2022 at 9:46 AM


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