3D cell culture plates and dishes with ultra-low attachment

To aid in cell culture research, PHC offers superior quality three-dimensional cell culture platforms with an array of well shapes that facilitate spheroid culturing of any specific cell type.

PrimeSurface cell culture labware are ultra-low attachment (ULA) plates and dishes that allow for scaffold-free, self-assembly of spheroid formation. The ULA plates facilitate the spontaneous spheroid formation of uniform shape and size because they are pre-coated with a distinct ultra-hydrophilic polymer.

The ULA plates are well suited for confocal microscopy and bright field imaging because of their high optical clarity. 96 well plates are available in V and M-bottom, as well as in the widely used 96 well U-bottom plate. This gives researchers options, allowing them to form tighter spheroids when required for specific cell types.

Additionally, 384 well plates are available in white and clear for high throughput screening (HTS) requirements.

Key benefits

  • Non-binding surface for cells to enable the natural spheroid formation
  • Spheroid assay formation and analysis in the same plate
  • Uniform single spheroid/EB formation in each well
  • High optical clarity plates for imaging
  • An array of well bottom shapes in 96 well formats, including V-bottom, Spindle-bottom, U-bottom
  • 384 well formats for high-throughput assay
  • Compatible with liquid robotic systems
  • Easy to handle
  • Non-cytotoxic, stable and cell non-adhesion surface
  • Compatible with fluorescence and bright-field imaging systems
  • White plates compatible with luminescent assays

The PrimeSurface series effectively prevents cell attachment without material and cytotoxic degradation because they are coated with a distinct ultra-hydrophilic polymer, covalently bound to the plastic surface.

The cutting-edge manufacturing processes and coating technologies facilitate the formation of uniform spheroid/EB with smooth surfaces, allowing clear cell images to be captured.

3D cell culture plates and dishes with ultra-low attachment

Image Credit: PHC Europe B.V.

The three different well bottom shapes of PrimeSurface 96 well plate

3D cell culture plates and dishes with ultra low attachment

Retinal tissue formation from Human ES Cells using PrimeSurface 96 well V-bottom plate

Image Credit: PHC Europe B.V.

3D cell culture plates and dishes with ultra low attachment

Cell culture from human caucasian hepatocyte carcinome using PrimeSurface 35ø dish

Image Credit: PHC Europe B.V.

3D cell culture plates and dishes with ultra low attachment

Three different well bottom shapes of PrimeSurface 96 well plate

Image Credit: PHC Europe B.V.

3D cell culture plates and dishes with ultra low attachment

Image Credit: PHC Europe B.V.

The new PrimeSurface 96 slit-well plate

To aid in cell culture research, PHC presents a new slit-well, ultra-low attachment 3D plate that facilitates easy handling of media exchange without disturbing spheroid formation.

Cell culturing requires regular media replacement to ensure optimum growing conditions for cells.

In a standard 96-well ultra-low cell attachment plate, media dispensing or aspiration is time-consuming as it must be carried out with extreme care to avoid disrupting the unattached spheroid.

Therefore, by using the PrimeSurface 96 Slit-Well Plate, media exchange for 96 well plates can be handled efficiently with one-step aspiration or dispensing for all 96 wells. The PrimeSurface 96 Slit-Well Plate can reduce pipetting time by more than 80% while also reducing the risk of damaging the spheroid.

PHC’s cell culture plates have numerous advantages, including the ability to undertake media exchange without disrupting the spheroid formation. When using these plates, media exchange time can also be reduced through the simultaneous delivery of cell culture media to all 96 wells.

Moreover, users can form and maintain uniform spheroids. The plates also hold a media capacity that is 1.5 times larger than conventional plates, reducing the number of media changes and increasing available nutrients.

With a well-engineered, time-saving design, the Slit-Well structure permits the concurrent dispensing of cell culture medium to all 96 wells.

3D cell culture plates and dishes with ultra low attachment

Image Credit: PHC Europe B.V.

Time Saving Design

The slit-Well structure allows for the simultaneous delivery of cell culture medium to all 96 wells.

Users also have the ability to form bigger spheroids in the same well for long-term cultures, but it is important to note that in order to produce larger spheroids, additional media is required. Slit-Well plates can use up to 1.5 times more media than conventional plates, thus delivering more nutrients to facilitate the growth of larger spheroids.

Conventional product: medium is independent in each well.

Conventional product: medium is independent in each well. Image Credit: PHC Europe B.V.

Slit-Well plate: medium is shared between wells.

Slit-Well plate: medium is shared between wells. Image Credit: PHC Europe B.V.

 

About PHCi

Founded in 1990 as a subsidiary of the PHC Holdings Corporation, it is our mission to become a leading, trusted brand for sustainable healthcare and biomedical product solutions, which support the work of our customers to improve the health and well-being of people around the world.

For more than 25 years now, we respond to the needs of our pharmaceutical, biotechnology, hospital/clinical and industrial customers, offering an unique perspective on scientific research in general. As a result we play a critical role in product development for worldwide applications and have established a reputation as a manufacturer of high-quality and innovative medical and laboratory equipment.

Long lasting relationships have been built with leading pharmaceutical, healthcare and biotechnology companies as well as with major academic and research institutes in Europe. PHC Europe B.V. has set the standard in many aspects. V.I.P. panels, Cool Safe compressors, Active Background Contamination Control and the world’s first -152°C ULT freezer. Where PHC Europe B.V. took the initiative, the others followed. This made us a very important player in both the ultra-low temperature and the CO2 market.


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Last updated: Dec 1, 2022 at 10:04 AM

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