The new BI-4500 SPR system provides multiple channel flow modes and delivers high quality binding response for low immobilization and small molecule (<100 Da) detection. Equipped with BI-DirectFlowTM technology, the BI-4500 system integrates precision sample delivery with near-zero dispersion for fast kinetics and effective removal of various secondary effects. Its modular innovative design gives users with optimal flexibility to choose amongst various analysis modules for life science, electrochemistry, and sensing in liquid and gas phase SPR applications.
The 5-channel Surface Plasmon Resonance (SPR) instrument, combined with the BI Autosampler, is the ideal solution for high throughput assay development. With the BI-DirectFlow™ technology, it provides precise sampler delivery and high quality binding kinetic measurements.
Key Features
- High Throughput with 5 Channels and Fully Automated Autosampler
Provides 5 channel flow modes and high sample capacity with up to two 384 well plates.
- High Sensitivity
Delivers high quality binding response for low immobilization and small molecule (< 100 Daltons) detection.
- Precise Sample Delivery with BI-DirectFlow™ Technology
Delivers sample with near-zero dispersion for fast kinetics and effective removal of various secondary effects.
- Temperature Controlled SPR Detection Module
Covers wide SPR angle range for measuring SPR shift in liquid phase. Precision background and reference subtraction. Temperature control for thermodynamic studies, 6 °C to 50 °C.
- Innovative Multi-Module Design
Provides users with maximum flexibility to choose amongst various analysis modules for electrochemistry and liquid applications.
Analysis Modules
Included:
- BI-DirectFlow™ Module for advanced five-channel ultra-fast kinetics research
Optional Accessories:
- EC-DualFlow™ Module for advanced two-channel electrochemical (EC) flow SPR research
- EC-SPR Module for electrochemical (EC) SPR research
- Gas SPR Module for gas phase and chemical vapor SPR research
- Autosampler Automate your BI SPR experience compatible with 384, 96, 48, and 12 plate formats
- BI Sensor Chips with high uniformity for reproducible SPR research
System Specifications
| Base Station |
Light source |
670 nm |
| Detection speed |
4 ms |
| Incident angles |
40-47 Deg (gas)
67-81 Deg (liquid) |
| Baseline noise |
< 0.06 RU RMS (0.01 mDeg RMS) |
| Baseline drift |
0.30 RU/hr (0.05 mDeg/hr) (when ambient drifts < 1 °C/hr) |
| Temperature Control Range |
6 °C to 50 °C (10 °C below ambient temperature max) |
| PC interface |
USB 3.0 |
| Outer dimension |
355(w) x 250 (h) x 515 (d) mm |
| Weight |
11.5 kg |
| Power supply |
110-230 V 50/60 Hz |
| Fluid Handling |
Number of sample flow channels |
5 channels |
| Flow cell material |
PEEK (biologically compatible) |
| Flow rate |
1.0 to 250 μL/min (application dependent) |
| Sample injection volume |
>50 μL (application dependent) |
| Sample injection method |
Fully automated (auto sampler option)
Semi-automated |
| Channel volume |
< 32 nL |
| Injection rise time |
< 0.2 s |
| Kinetic constant |
ka <1 X 108 M-1 s-1
kd >1 X 10-6 s-1 |
| Affinity |
KD = 10-3 M (1 mM) to 10-2 M (1 pM) |
| Molecular weight cutoff |
100 Da |
| Analysis module |
5 channel BI-DirectFlowTM Module |
| Control System |
Computer |
Windows operating system |
| Software |
BI-SPR software including Data Analysis and Kinetics Analysis packages |
Applications
Life Science
Our Flow injection technique and analysis modules can help you with drug discovery and development, protein-protein/ protein-DNA/ protein-drug interaction studies, gene assays, immunosensing, and more.
Electrochemistry
Our Electrochemical SPR technique and analysis modules can help you with a variety of electro-analytical applications such as anodic stripping analysis, electropolymerization, electrodeposition, redox-related conformational change, and more.
Chemical Vapor Sensing
Our Gas Phase SPR technique and analysis modules can help you study fundamental solid-gas interface interactions, detect toxic molecules in the environment, perform various chemical analysis for medical research, and more.
Environment, Food & Beyond
Our BI SPR techniques can help you with broader applications such as food safety, quality control, environmental monitoring, or the development of new high-performance and coupled SPR techniques.