BlotGlyco® O-Glycan Sample Preparation Kit is a streamlined process for releasing and purifying O-glycans for detection by HPLC, LC-MS, MALDI-TOF MS.

  • BlotGlyco® O-Glycan Sample Preparation Kit are used for O-glycan analysis in a broad range of biological samples such as purified glycoproteins and crude samples such as unpurified glycoproteins, serum, urine, cell and tissue lysates and plant extracts.
  • The kit allows flexibility for choice of label* with detection methods including HPLC, LC-MS, MALDI-TOF MS or CE.
  • BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of O-glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities.

*label not included with the kit

MSDS for BlotGlyco® Bead
MSDS for O-Glycan releasing reagent

Application

  • Bioprocessing and biomarker O-glycan analysis.
  • Glycosylation analysis of biologics for research, screening, quality control and production process development and monitoring.
  • O-Glycosylation analysis of biological samples such as mucins in cancer research.

Features

  • BlotGlyco® O-Glycan Sample Preparation Kit contains mild ammonium salt for the releasing reaction, which is a safe and simplified protocal over hydrazinolysis.
  • The kit provides a rapid, reliable and reproducable results for O-glycan sample preparation.
  • Uses simple lab equipment such as SpeedVac, heating block and centrifuge.
  • Contains nontoxic chemicals.

Other Equipment by this Supplier

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.