The DAWN® multi-angle light scattering (MALS) detector connects to most HPLC, GPC, FPLC or FFF separation systems to determine absolute molecular weight, size, and conformation of peptides, proteins, polymers and nanoparticles, in solution, without reference to calibration standards.
Eliminate column calibration and idealized assumptions, gain certainty with first-principles MALS analysis. With 18 detection angles, superb optical engineering and a range of optional add-ons, the DAWN provides the highest sensitivity, range of measurement and versatility of any available MALS detector.
Features
- Sensitivity in SEC-MALS mode: 10 ng of 100 kDa polystyrene in THF, or 200 ng of BSA in PBS, using 7.8 x 300 mm SEC columns
- Range of molar mass: 200 Da to 1 GDa (may vary with molecular composition or solvent)
- Range of rms radius Rg : 10 nm to 500 nm (up to 1000 nm with specific shape models)
- Temperature-controlled models: ambient; H/C, -15°C to +150°C; UHT, room temperature to +210°C
- Integrated ultrasonic cell cleaning module
- DLS: optional WyattQELS™ embedded dynamic light scattering detector for particle sizes from 0.3 nm to 300 nm (Rh)
- Optional fluorescent-molecule configuration includes infrared laser and fluorescence-blocking filters
- Touch-panel display for immediate access to data, diagnostics and settings
- Serviceability: fully field-serviceable with extended self-diagnosis of field-replaceable flow cell and hardware modules
- Software: ASTRA® chromatography software provides comprehensive analyses and user-friendly features including HPLC control, “One-click MW” and user-designed reports. Optional 21CFR11 support for GMP.
Applications
- Proteins: absolute molar mass, size and stoichiometry of monomers, oligomers, complexes, aggregates and fragments.
- Glycoproteins, detergent-solubilized membrane proteins, PEGylated proteins, protein-polysaccharide and protein-DNA complexes; heme-containing proteins that absorb laser light
- Protein-protein and protein-nucleic acid interactions
- Polymers: absolute molar mass and size (distributions and moments), conformation, branching and second virial coefficient
- Linear or branched polymers, co-polymers, fluorescent polymers such as lignin
- Nanoparticles: size distributions, structure, encapsulation and concentration of vesicles, liposomes, dendrimers, viruses, VLPs, fibrils and more
- Most aqueous and organic solvents