The FDSS/μCELL is a kinetic plate reader with an integrated dispensing head and imaging-based detector.
Simultaneous dispensing into the entire 96/384-well plates and simultaneous detection of the kinetics of the fluorescence or luminescence intensity allow quick measurements with no time lag for the 96/384-well format.
The technologies employed in the FDSS series are integrated into a compact body, enabling simple-touse operation, suitable for assay development or in research basic cell-based kinetic assays.
Features
- Whole Plate Dispensing and Imaging
- Uniform conditions and no time lag for live cell measurements
- Reduced measurement time
- Simultaneous dispensing into the whole 96/384 plates
- Simultaneous detection of the kinetics of intensity changes
- Compact and Simple-to-Use
- Simple to usc for all users
- Various data output and analysis functions
- Versatile and Specially Designed System Structure
- High precision wth whole plate simultaneous dispensing (CV below 5%)
- Disposable 961384 dispensing tips
- Dispensing of multiple compounds possible
- Wash and wipe functions (laclory bu41-in option)
"96 wells <5% CV al 10 μL, 384 Well <5% CV al 5 μL
- Specially designed optics to detect the whole plate with high uniformity
- Specially designed illumination optics for fluorescence
- Long life-time light source with selectable excitation wavelength (4eo nm «530 rm)
- Motionless assay plate reducing the damages to live cells
- Proprietary imaging detectors allowing high-sensitivity measurement
Applications
- Calcium Assay with Fluo-4 (1)
- Calcium Assay with Fluo-4 (2)
- Normal HEK293 cells stimulated by Carbachol
- Calcium measurement of H1 NFAT HEK293 by BacMam Aequorin from Invitrogen
- Cardiomyocyte calcium oscillation
- Kinetic analysis of endogenous b2AR full and partial agonists in HEK293 cAMP GloSensor cell line from Promega
Specifications
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Type number
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FDSS/uCELL
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Dispenser
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96-tip type: 10 μL to 200 μL
384-tip type: 1 μL to 30 μL
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Sensor
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High-speed, high-sensitivity digital CCD camera for fluorescence
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Sampling time (Interval)
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0.5 s to 100 s/Data point
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Light source optics for intracellular ion measurement
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480 nm excitation and 540 nm emission
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Plate handling
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Assay plate ×1 and compound plate ×2
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Adaptable microplate
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Clear bottom black plate
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Tip/Plate loading
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Manual loading
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Maximum sampling data point
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4000
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Dimension/weight
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550 mm (W)×670 mm (D)×1600 mm (H)/Approx. 200 kg
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