The Instrument for Biomolecular Interaction Sensing MultipleX 96 (IBIS-MX96) is a comprehensive system for multiplex biomolecular interaction imaging.
The MX96 is optimized for monitoring 96 ligand spots on a sensor surface, which are deposited from flow with our CFM Printer. Using the MX96's temperature controlled 96 well autosampler, analytes are injected in series across the chip, yielding 96 binding interactions for each injection. It is possible to perform unattended overnight or weekend runs from a 96 well plate, thereby generating up to 9,200 sensorgrams in a full run.
The MX96 utilizes a proprietary back-and-forth flow for sample injection, enabling long association times with only 80 uL of sample!
Because the ligand deposition process is done using the CFM's enclosed microchannels, each ligand spot can have a different concentration, buffer composition, pH, detergent mix, and so forth. Thus, 96 different conditions can be assayed in a single step, while the protein of interest is still active. For example, in our work with studying GPCR solubilization conditions, we optimized the solubilization conditions for CXCR5 in 2 hours, while the same process would have taken 2 weeks on a standard SPR biosensor (see Myszka et al, "Detergent Screening..." in Technology/Publications).