CellTiter-Glo® 2.0 Cell Viability Assay for for fast, easy, everyday use

The CellTiter-Glo® 2.0 Cell Viability Assay is a luminescent method for swift, convenient, routine cell viability assessments.

  • Offers equivalent performance to the classic CellTiter-Glo® Cell Viability Assay
  • ATP assay to identify living cells
  • Shorter preparation times and easy repurposing over several days

New! Use with the New MyGlo Reagent Reader from Promega. A customized 96-well MyGlo reagent reader system incorporating instrument, assay preparation, and data processing will help users speed up and simplify cell viability tests. MyGlo detects more than just assay signals. It walks users through every step of the CellTiter-Glo® experiments.

CellTiter-Glo® 2.0 measures ATP, a key indicator of cell health

CellTiter-Glo® 2.0, built upon the foundational chemistry of the original CellTiter-Glo® Cell Viability Assay, features a single-reagent system with enhanced stability, simplifying storage and setup. Whether conducting cell proliferation assays on a small scale or handling numerous plates concurrently, this ready-to-use reagent streamlines daily operations. It is suitable for high-throughput screening and multiplex applications with its heightened sensitivity and wide linear range.

The CellTiter-Glo® 2.0 Assay gauges the number of viable cells in culture by measuring ATP levels as a marker for metabolically active cells. The luminescent signal obtained directly correlates with the abundance of viable cells in the culture.

Simple assay setup and enhanced stability

With a single, pre-prepared reagent and a straightforward "add-mix-measure" process, there is no requirement for mixing individual components.

The CellTiter-Glo® 2.0 reagent maintains stability both at room temperature and at 4 °C, facilitating rapid and effortless assay execution while eliminating the need for thawing and mixing, as required by alternative ATP assays. This enhanced stability also enables convenient storage.

CellTiter-Glo® 2.0 Cell Viability Assay for for fast, easy, everyday use

Image Credit: Promega Corporation

Source: Promega Corporation

Percent Activity Remaining CellTiter-Glo® 2.0 Reagent Stability CellTiter-Glo® "Original" Reagent Stability
>85% Activity at 22 °C 7 days 12 hours
>85% Activity at 4 °C 2 months 3.5 days

 

Assay performance

The CellTiter-Glo® 2.0 Cell Viability Assay is appropriate for high-throughput screening and benchtop applications due to its superior performance compared to the original CellTiter-Glo® Assay.

High sensitivity and broad linearity. Luminescence measured with the CellTiter-Glo® 2.0 Assay is proportional to the number of viable Jurkat cells in culture over three orders of magnitude in 96-well, 384-well, and 1536-well plates.

High sensitivity and broad linearity. Luminescence measured with the CellTiter-Glo® 2.0 Assay is proportional to the number of viable Jurkat cells in culture over three orders of magnitude in 96-well, 384-well, and 1536-well plates. Image Credit: Promega Corporation

Multiplex with other cell viability or cytotoxicity assays

Multiplexing the CellTiter-Glo® 2.0 cell viability assay with other assays can decrease labor and costs associated with cell culture. Complementary measures of cell health can be used to compensate for mistakes in plating, culturing, or assay chemistry interferences by multiplexing assays in the same sample well.

In the provided example, bortezomib-treated K562 cells were exposed for 48 hours before the addition of CellTox Green cytotoxicity assay reagent. The fluorescence associated with cytotoxicity was then measured. The CellTiter-Glo® 2.0 assay reagent was introduced, and luminescence was measured to assess cell proliferation.

CellTiter-Glo® 2.0 Cell Viability Assay for for fast, easy, everyday use

Image Credit: Promega Corporation

Specifications

What is in the box?

Source: Promega Corporation

Item Part # Size
CellTiter-Glo® 2.0 Reagent G924A 1 × 10ml

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