A portable device similar to today's home pregnancy tests that can quickly detect the presence of infectious diseases, including HIV-AIDS and measles, as well as biological agents such as ricin and anthrax, is the object of a new joint university/industry research project.
Vanderbilt University's Institute for Integrative Biosystems Research and Education (VIIBRE) and Pria Diagnostics LLC, a privately held California company that specializes in miniaturized medical diagnostics, agreed to collaborate on the development before the holidays.
VIIBRE has spent the last three years developing the ability to measure the metabolism of small groups of cells and studying how they respond to drugs, toxins and pollutants. To do so, the interdisciplinary team has developed two basic technologies: special electrodes that can measure the concentrations of the chemicals that cells consume and excrete in extremely small volumes and the use of fluids flowing through microscopic channels to move and manipulate small numbers of cells reliably. In the process, the group has applied for more than 12 patents.
Meanwhile, Pria has developed a micro-optical fluorescence spectroscopy system and used it as the basis for a inexpensive male fertility detector that can be used in the home to measure sperm motility with an accuracy comparable to laboratory analyses.
"I'm thrilled at how well the VIIBRE and PRIA technologies mesh," says John P. Wikswo, professor of biomedical engineering, physiology and physics at Vanderbilt and director of VIIBRE. "We are already making rapid progress on prototyping portable instruments for clinical diagnosis and biodefense."
"Today the treatment for AIDS is very expensive and there is always a question about when to start and stop anti-retroviral therapy," says Pria's Chief Technology Officer Jason Pyle. "We are developing a device that we hope will allow medical professionals and HIV patients to manage their disease in a way that is similar to how diabetes patients can monitor their condition since the introduction of home blood glucose detectors." The collaboration's goal is to produce its first portable HIV monitor within two years. In addition to such "point of care" devices, Wikswo and Pyle are joining forces to develop "high-throughput" screening systems that can determine the biological activity of large numbers of compounds with extreme rapidity and so could have a major impact on the drug discovery process.
Fifteen years ago a number of start-up companies were created to make the goal of creating a "lab-on-a-chip" a reality. However, putting microscopic arrays of channels, pumps and valves to move around minute amounts of liquid on silicon chips proved to be considerably more difficult than most of the inventors had expected and the products that these companies have created thus far have been too expensive for the point-of-care diagnostics market.
For their home fertility tester, Pria kept costs down by keeping their system as simple as possible. Instead of trying to squeeze everything onto a single chip, Pria designers started with a desktop diagnostic system and shrank it down into a device about the size of a coffee cup. One of the cost-saving aspects of the design was to keep the fluid-handling components separate from the microelectronics. The resulting device is considerably larger than comparable lab-on-a-chip systems but it is also much less expensive. "That's one of the appealing features about Pria's approach," says Wikswo, "They keep their microfluidics and microelectronics as simple as possible."
"Pria's first product focused on fertility," says VIIBRE project engineer David Schaffer. "With our capabilities, they can begin applying their technology to a goldmine of different applications."
One of the key VIIBRE capabilities, which was developed by a research team headed by Assistant Professor of Chemistry David Cliffel, is the development of a sensor suite capable of simultaneously measuring the concentrations of the key chemicals that cells consume and excrete--oxygen, glucose and lactic acid--with enough sensitivity to monitor the health of a few thousand cells confined in a small volume. (For more detail see "New device can help defend against novel biological agents" at [http://exploration.vanderbilt.edu/news/news_microphys.htm].)