Anti-idiotypic antibodies (anti-ID Ab) are antibodies that identify and attach to the idiotypic center of other antibodies. Anti-idiotype antibodies are commonly employed in therapeutic drug development, such as immunogenicity evaluations and PK/PD studies.
Sino Biological offers a comprehensive spectrum of bespoke anti-idiotypic antibody manufacturing services, including antigen preparation, anti-idiotypic antibody development, detection method establishment, and kit development.
The company has expertise in high-throughput antibody expression and screening, as well as enzymatic cleavage and purification of diverse antibody formats.
The anti-ID antibodies exhibit excellent titer specificity and little cross-reactivity with human IgG. Anti-idiotypic antibodies, both polyclonal and monoclonal, can be created to aid in drug development, preclinical research and clinical trials.
Types of Anti-idiotypic Antibodies. Anti-idiotypic antibodies are tailored to your needs and can be used to set up PK assays in different formats to determine free or total drug concentrations in samples. Image Credit: Sino Biological Inc.
Service highlights
Diverse modality targeting
F(ab’)2, Fab, scFv, ADC, bsAb, Fc-fusion protein, and other antibodies are available.
Superior performance
Serum ELISA titer ≥1:729,000; 2% cross-reactivity of pure Abs with total IgG; >99% success rate.
Multiple immunization strategies
High screening success rates and increased antibody titers
Various purification methods
Protein A, antigen affinity, total human IgG, Isotype IgG, and more.
Anti-idiotypic antibody packages
Anti-ID rabbit pAb production service
Time: 2–3 months
Service details
- Antigen validation (1–2 days)
- Antigen preparation (1–2 weeks, optional)
- Full-length IgG is fragmented and purified to produce F(ab) and F(ab’)2 fragments.
- Immunization (8-10 weeks)
- Pre-immune bleed
- Rabbit immunization
- Serum titer test
- Final bleed
- Purification (2–3 weeks)
- Protein A purification
- Antigen affinity purification
- Total IgG purification (optional)
- Isotype IgG purification (optional)
- QC analysis (1 week)
- SDS-PAGE and UV analysis
- ELISA identification
Deliverables
Anti-ID mouse mAb production service
Time: 4–6 months
Service details
- Antigen validation (1–2 days)
- Antigen preparation (1–2 weeks, optional)
- F(ab) and F(ab’)2 fragments were obtained by fragmenting and purifying full-length IgG.
- Immunization (6–10 weeks)
- Pre-immune bleed
- 5-10 Balb/c mouse immunization
- Serum titer test
- Fusion and Selection (8–11 weeks)
- Fusion and screening
- Competitive ELISA to identify clones with blocking potency (optional)
- Subcloning and cell expansion
- Cell banking
- Ab production and purification (2–3 weeks)
- Protein A purification
- Hybridoma cell culture at the required scale
- QC analysis (1 week)
- SDS-PAGE and UV analysis
- ELISA identification
- ELISA pairing (2–3 weeks, Optional)
- Antibody HRP labeling and ELISA pairing
- Sensitivity analysis of anti-ID mAb pair
Deliverables
- Positive clones, 2 tubes cells/clone
- Purified antibodies, 10 mg/clone (or as requested)
- ELISA antibody pair (optional)
- CoA
Anti-ID rabbit mAb production service
Time: 4–6 months
Service details
- Antigen validation (1–2 days)
- Antigen preparation (1–2 weeks, optional)
- Full-length IgG is fragmented and purified to produce F(ab) and F(ab’)2 fragments.
- Immunization (8–10 weeks)
- Pre-immune bleed
- Two rabbits immunization
- Serum titer test
- Final bleed
- Library construction and screening (4–6 weeks)
- Phage library construction and evaluation
- Screening phage libraries and clone identification
- Ab production and QC analysis (2–3 weeks)
- Vector construction
- Transient transfection of HEK293 cells
- Protein A purification
- SDS-PAGE and UV analysis
- ELISA validation
- ELISA pairing (2–3 weeks, optional)
- Antibody HRP labeling and ELISA pairing
Deliverables
- Antibody H and L chain sequences
- CoA
- Anti-ID pair screening (optional)
- Purified antibodies
- H and L chains in separate expression vectors
Purity >90%, concentration >1 mg/mL, and PBS formulation are all requirements for client-supplied antigens.
Case studies of custom anti-idiotypic antibody development service
Case #1: Anti-idiotypic mAb generation
Sino Biological used the hybridoma technique to produce and identify nine anti-idiotype mouse monoclonal antibody clones. These antibodies had a strong specific affinity to the antibody-drug but no binding to human IgG isotype control or total human IgG.
Image Credit: Sino Biological Inc.
Case #2: Anti-idiotypic pAb generation
Drugs A and B were the targets of two anti-idiotype rabbit polyclonal antibodies. Anti-idiotype polyclonal antibodies purified in four steps exhibited a 2% cross-reactivity with human IgG.
Image Credit: Sino Biological Inc.