IFN-γ: A therapeutic target for cancer therapy

Interferon gamma (IFN-γ) is a cytokine that induces a series of immune responses against viruses and tumors. The pro-apoptotic, anti-tumor and anti-proliferative actions of the cells are activated when IFN-γ binds to the IFN-γ receptor, which is made up of IFNGR1 and IFNGR2.

IFN-γ expression that is erroneous encourages the growth of tumors. IFN-γ has thus become recognized as a major contributor to the immunogenicity of malignancy.

The CD4+ T helper cells and CD8+ cytotoxic T cells are the effector T cells that release IFN-γ. Natural killer cells, natural killer T cells, and B cells are additional immune cells that secrete IFN-γ.

IFN-γ is stimulated by interleukins such IL-12, IL-15, IL-18, and IL-21. These interleukins’ stimulatory effects also result from intracellular signaling of transcription factors such as AP-1, JAK/STAT, and T-bet.

In PD-1/PD-L1 immune checkpoint inhibitor cancer treatment, IFN-γ is a prognostic marker. The production of IFN-γ is associated with an improved response to immune checkpoint blockade (ICB) therapy in triple-negative breast cancer, and is considered to be an emerging high-potential adjuvant therapy target.

Recombinant IFN-γ and Receptor Proteins

To facilitate the study of IFN- in cancer immunity, Sino Biological has produced a panel of recombinant IFN-γ protein and receptor reagents.

Featured IFN-γ and Receptor Proteins

Human IFN-γ Protein Cat#: 11725-HNAE

  • Tag-Free
  • HPLC-verified
  • High Activity
  • High-Purity

IFN-γ: A therapeutic target for cancer therapy

Purity: ≥ 95% as determined by SDS-PAGE & HPLC. Image Credit: Sino Biological Inc.

IFN-γ: A therapeutic target for cancer therapy

Anti-viral assays using WISH cells infected with vesicular stomatitis virus (VSV). Image Credit: Sino Biological Inc.

Human IFNGR1 Protein Cat#: 10338-H08H

IFN-γ: A therapeutic target for cancer therapy

Ability to inhibit rhIFN-γ mediated protection of WISH cells infected with vesicular stomatitis virus (VSV). Image Credit: Sino Biological Inc.

Mouse IFN-γ Protein Cat#: 50709-MNAH

IFN-γ: A therapeutic target for cancer therapy

Immobilized Mouse Interferon Gamma can bind Mouse IFNGR1 (Cat: 50705-M02H). Image Credit: Sino Biological Inc.

IFN-γ: A therapeutic target for cancer therapy

Antiviral assays using L929 cells infected with vesicular stomatitis virus (VSV). Image Credit: Sino Biological Inc.

Human IFNGR2 Protein Cat#: 50665-M08H

IFN-γ: A therapeutic target for cancer therapy

Purity: > 97 % as determined by SDS-PAGE. Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method. Image Credit: Sino Biological Inc.

Product list of Recombinant IFN-γ & Receptors

Table 1. Source: Sino Biological Inc.

Cat# Molecule Species Expressed Host Purity Activity Tag
11725-HNAE IFNG Human E. coli ≥95 % (by SEC-HPLC) Active Native
11725-HNAS Human CHO Stable Cells >92% Active Native
50709-M02H Mouse HEK293 Cells >92% Active C-hFc
50709-M08H Mouse HEK293 Cells >75% Active C-His
50709-MNAH Mouse HEK293 Cells >85% Active Native
60007-F08H Ferret HEK293 Cells >95%   C-His
80234-R02H Rat HEK293 Cells >92% Active C-hFc
90008-C08H Rhesus HEK293 Cells >90% Active C-His
90008-CNAE Rhesus E. coli >90% Active Native
10338-H02H IFNGR1 Human HEK293 Cells >95%   C-hFc
10338-H08H Human HEK293 Cells >97% Active C-His
50705-M02H Mouse HEK293 Cells >95% Active C-hFc
50705-M08H Mouse HEK293 Cells >98% Active C-His
80182-R02H Rat HEK293 Cells >95% Active C-hFc
90207-C02H Cynomolgus HEK293 Cells >95% Active C-hFc
90207-C08H Cynomolgus HEK293 Cells >95%   C-His
50665-M08H IFNGR2 Mouse HEK293 Cells >97%   C-His

 

Other related proteins

  • PD-1
  • CD4
  • IL-12
  • PD-L1
  • IL-18
  • CD8
  • IL-15
  • IL-21

Antibodies for IFN-γ and Receptors

For use in ELISA, WB, FCM, and IHC, Sino Biological has created a panel of antibodies that target the IFN-γ cytokine and its receptors.

Anti-IFN-γ Antibody (FITC), Rabbit Mab Cat#: 11725-R003-F

IFN-γ: A therapeutic target for cancer therapy

Flow cytometric analysis of Human IFN-γ expression on human peripheral blood lymphocytes. Image Credit: Sino Biological Inc.

Anti-IFN-γ Antibody, Rabbit PAb Cat#: 105908-T08

IFN-γ: A therapeutic target for cancer therapy

Immunochemical staining of human IFNG in human kidney cell. Image Credit: Sino Biological Inc.

Anti-IFNGR1 Antibody (APC), Mouse MAb Cat#: 10338-MM05-A

IFN-γ: A therapeutic target for cancer therapy

Flow cytometric analysis of Human IFNGR1 expression on human whole blood lymphocytes. Image Credit: Sino Biological Inc.

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